ArticlePloS one2013
RiboTag analysis of actively translated mRNAs in Sertoli and Leydig cells in vivo.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Article
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- Repeated fentanyl abstinence intensifies opioid withdrawal and induces a proinflammatory state in striatal microglia.bioRxiv : the preprint server for biology · 2025Article
- In vivo profiling of the endothelium using 'AngioTag' zebrafish.Angiogenesis · 2025Article
- Androgens inhibit protective CD8Nature communications · 2025Article
- Review
- Review
- Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Orphan G Protein-Coupled Receptor GPR37 as an Emerging Therapeutic Target.ACS chemical neuroscience · 2023Review
- Inducible CRISPR Epigenome Systems Mimic Cocaine Induced Bidirectional Regulation of Nab2 and Egr3.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Functions of Steroid Hormones in the Male Reproductive Tract as Revealed by Mouse Models.International journal of molecular sciences · 2023Review
- Decoding the Spermatogenesis Program: New Insights from Transcriptomic Analyses.Annual review of genetics · 2022Review
- Revisiting the Characteristics of Testicular Germ Cell Lines GC-1(spg) and GC-2(spd)ts.Molecular biotechnology · 2021Article
- Estrogen Regulation of the Molecular Phenotype and Active Translatome of AVPV Kisspeptin Neurons.Endocrinology · 2021Article
- Kiss1 is differentially regulated in male and female mice by the homeodomain transcription factor VAX1.Molecular and cellular endocrinology · 2021Article
- Androgen Actions in the Testis and the Regulation of Spermatogenesis.Advances in experimental medicine and biology · 2021Review
- Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells.Frontiers in cell and developmental biology · 2021Article
- Characterization of rodent Sertoli cell primary cultures.Molecular reproduction and development · 2020Review
- Follicle-stimulating Hormone (FSH) Action on Spermatogenesis: A Focus on Physiological and Therapeutic Roles.Journal of clinical medicine · 2020Review
- Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors.Nature cell biology · 2020Article
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Authors and funding
9 authors.
Funding
Abstract
Male spermatogenesis is a complex biological process that is regulated by hormonal signals from the hypothalamus (GnRH), the pituitary gonadotropins (LH and FSH) and the testis (androgens, inhibin). The two key somatic cell types of the testis, Leydig and Sertoli cells, respond to gonadotropins and androgens and regulate the development and maturation of fertilization competent spermatozoa. Although progress has been made in the identification of specific transcripts that are translated in Sertoli and Leydig cells and their response to hormones, efforts to expand these studies have been restricted by technical hurdles. In order to address this problem we have applied an in vivo ribosome tagging strategy (RiboTag) that allows a detailed and physiologically relevant characterization of the "translatome" (polysome-associated mRNAs) of Leydig or Sertoli cells in vivo. Our analysis identified all previously characterized Leydig and Sertoli cell-specific markers and identified in a comprehensive manner novel markers of Leydig and Sertoli cells; the translational response of these two cell types to gonadotropins or testosterone was also investigated. Modulation of a small subset of Sertoli cell genes occurred after FSH and testosterone stimulation. However, Leydig cells responded robustly to gonadotropin deprivation and LH restoration with acute changes in polysome-associated mRNAs. These studies identified the transcription factors that are induced by LH stimulation, uncovered novel potential regulators of LH signaling and steroidogenesis, and demonstrate the effects of LH on the translational machinery in vivo in the Leydig cell.
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