ArticlePloS one2013
Hypo- and hyperglycemia impair endothelial cell actin alignment and nitric oxide synthase activation in response to shear stress.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Preclinical techniques to investigate exercise training in vascular pathophysiology.American journal of physiology. Heart and circulatory physiology · 2021Pooled it
- A randomised study of the impact of the SGLT2 inhibitor dapagliflozin on microvascular and macrovascular circulation.Cardiovascular diabetology · 2017 · on this mapTrial
- A Link Between Hypoglycemia and Progression of Atherosclerosis in the Veterans Affairs Diabetes Trial (VADT).Diabetes care · 2016Trial
- The changing epidemiology of human type 2 diabetes-associated atherosclerosis: Pathophysiological mechanisms and emerging treatment possibilities.Journal of internal medicine · 2026Review
- Glutamine enhances endothelial cell survival and vasodilation by increasing glutathione to reduce oxidative stress.Physiological reports · 2026Article
- Protein kinase C and endothelial dysfunction in select vascular diseases.Frontiers in cardiovascular medicine · 2025Review
- Acute severe hypoglycemia alters mouse brain microvascular proteome.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024Article
- The Specific Molecular Changes Induced by Diabetic Conditions in Valvular Endothelial Cells and upon Their Interactions with Monocytes Contribute to Endothelial Dysfunction.International journal of molecular sciences · 2024Article
- Experimental Models to Study the Functions of the Blood-Brain Barrier.Bioengineering (Basel, Switzerland) · 2023Review
- SIRT3 attenuates coronary atherosclerosis in diabetic patients by regulating endothelial cell function.Journal of clinical laboratory analysis · 2022Article
- Confocal imaging dataset to assess endothelial cell orientation during extreme glucose conditions.Scientific data · 2022Article
- Laminar Flow on Endothelial Cells Suppresses eNOS O-GlcNAcylation to Promote eNOS Activity.Circulation research · 2021Article
- Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations.Scientific reports · 2021Article
- Review
- Hyperglycemia and hyperlipidemia can induce morphophysiological changes in rat cardiac cell line.Biochemistry and biophysics reports · 2021Article
- Endothelial response to glucose: dysfunction, metabolism, and transport.Biochemical Society transactions · 2021Review
- Mechanical Forces Regulate Asymmetric Vascular Cell Alignment.Biophysical journal · 2020Article
- Exposure to hypoglycemia and risk of stroke.Annals of the New York Academy of Sciences · 2018Review
- Interaction of diabetes and smoking on stroke: a population-based cross-sectional survey in China.BMJ open · 2018Article
- Functional differences between healthy and diabetic endothelial cells on topographical cues.Biomaterials · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uncontrolled blood glucose in people with diabetes correlates with endothelial cell dysfunction, which contributes to accelerated atherosclerosis and subsequent myocardial infarction, stroke, and peripheral vascular disease. In vitro, both low and high glucose induce endothelial cell dysfunction; however the effect of altered glucose on endothelial cell fluid flow response has not been studied. This is critical to understanding diabetic cardiovascular disease, since endothelial cell cytoskeletal alignment and nitric oxide release in response to shear stress from flowing blood are atheroprotective. In this study, porcine aortic endothelial cells were cultured in 1, 5.55, and 33 mM D-glucose medium (low, normal, and high glucose) and exposed to 20 dynes/cm(2) shear stress for up to 24 hours in a parallel plate flow chamber. Actin alignment and endothelial nitric oxide synthase phosphorylation increased with shear stress for cells in normal glucose, but not cells in low and high glucose. Both low and high glucose elevated protein kinase C (PKC) levels; however PKC blockade only restored actin alignment in high glucose cells. Cells in low glucose instead released vascular endothelial growth factor (VEGF), which translocated β-catenin away from the cell membrane and disabled the mechanosensory complex. Blocking VEGF in low glucose restored cell actin alignment in response to shear stress. These data suggest that low and high glucose alter endothelial cell alignment and nitric oxide production in response to shear stress through different mechanisms.
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