ArticlePloS one2013
Analysis of epigenetic factors in mouse embryonic neural stem cells exposed to hyperglycemia.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Article
- Research advances on the impact of gestational diabetes mellitus on offspring neurodevelopment.Diabetology & metabolic syndrome · 2026Review
- Maternal Diabetes Deregulates the Expression of Mecp2 via miR-26b-5p in Mouse Embryonic Neural Stem Cells.Cells · 2023Article
- Article
- Stem cell therapy for central nervous system disorders: Metabolic interactions between transplanted cells and local microenvironments.Neurobiology of disease · 2022Review
- Role of Diet in Stem and Cancer Stem Cells.International journal of molecular sciences · 2022Review
- Cerebral Effects of Neonatal Dysglycemia.Clinics in perinatology · 2022Review
- High glucose causes developmental abnormalities in neuroepithelial cysts with actin and HK1 distribution changes.Frontiers in cell and developmental biology · 2022Article
- Diabetes, Oxidative Stress, and DNA Damage Modulate Cranial Neural Crest Cell Development and the Phenotype Variability of Craniofacial Disorders.Frontiers in cell and developmental biology · 2021Review
- High glucose alters the DNA methylation pattern of neurodevelopment associated genes in human neural progenitor cells in vitro.Scientific reports · 2020Article
- Can miRNAs Be Considered as Diagnostic and Therapeutic Molecules in Ischemic Stroke Pathogenesis?-Current Status.International journal of molecular sciences · 2020Review
- Identification of histone acetylation markers in human fetal brains and increased H4K5ac expression in neural tube defects.Molecular genetics & genomic medicine · 2019Article
- Maternal hyperglycemia disturbs neocortical neurogenesis via epigenetic regulation in C57BL/6J mice.Cell death & disease · 2019Article
- Sensory Neuropathy Affects Cardiac miRNA Expression Network TargetingInternational journal of molecular sciences · 2019Article
- Diabetes in Pregnancy and MicroRNAs: Promises and Limitations in Their Clinical Application.Non-coding RNA · 2018Review
- Fasting Blood Glucose Level in Locally Advanced Non-Small Cell Lung Cancer: a New Prognostic Factor?Hormones & cancer · 2018Article
- Arsenic Induces Members of the mmu-miR-466-669 Cluster Which Reduces NeuroD1 Expression.Toxicological sciences : an official journal of the Society of Toxicology · 2018Article
- Maternal Diabetes and Fetal Programming Toward Neurological Diseases: Beyond Neural Tube Defects.Frontiers in endocrinology · 2018Review
- miR-210 expression is associated with methionine-induced differentiation of trout satellite cells.The Journal of experimental biology · 2017Article
- Maternal Factors that Induce Epigenetic Changes Contribute to Neurological Disorders in Offspring.Genes · 2017Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMaternal diabetes alters gene expression leading to neural tube defects (NTDs) in the developing brain. The mechanistic pathways that deregulate the gene expression remain unknown. It is hypothesized that exposure of neural stem cells (NSCs) to high glucose/hyperglycemia results in activation of epigenetic mechanisms which alter gene expression and cell fate during brain development. METHODS AND
findingsNSCs were isolated from normal pregnancy and streptozotocin induced-diabetic pregnancy and cultured in physiological glucose. In order to examine hyperglycemia induced epigenetic changes in NSCs, chromatin reorganization, global histone status at lysine 9 residue of histone H3 (acetylation and trimethylation) and global DNA methylation were examined and found to be altered by hyperglycemia. In NSCs, hyperglycemia increased the expression of Dcx (Doublecortin) and Pafah1b1 (Platelet activating factor acetyl hydrolase, isoform 1b, subunit 1) proteins concomitant with decreased expression of four microRNAs (mmu-miR-200a, mmu-miR-200b, mmu-miR-466a-3p and mmu-miR-466 d-3p) predicted to target these genes. Knockdown of specific microRNAs in NSCs resulted in increased expression of Dcx and Pafah1b1 proteins confirming target prediction and altered NSC fate by increasing the expression of neuronal and glial lineage markers. CONCLUSION/
interpretationThis study revealed that hyperglycemia alters the epigenetic mechanisms in NSCs, resulting in altered expression of some development control genes which may form the basis for the NTDs. Since epigenetic changes are reversible, they may be valuable therapeutic targets in order to improve fetal outcomes in diabetic pregnancy.
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