ArticlePloS one2013
Live-cell dynamic sensing of Cd(2+) with a FRET-based indicator.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- CadmiLume: A Novel Smartphone-Based Bioluminescence Color-Tuning Assay and Biosensor for Cadmium and Heavy Metal Detection in Water Samples.Methods and protocols · 2025Article
- CdACS omega · 2023Article
- Portable sensing devices for smart healthcare and prevention of lead poisoning.Journal of the Chinese Medical Association : JCMA · 2023Review
- Real-Time Monitoring of Selenium in Living Cells by Fluorescence Resonance Energy Transfer-Based Genetically Encoded Ratiometric Nanosensors.ACS omega · 2023Article
- An Overlooked Hepcidin-Cadmium Connection.International journal of molecular sciences · 2022Article
- Fluorescent indicators for live-cell and in vitro detection of inorganic cadmium dynamics.Journal of fluorescence · 2022Article
- Bioluminescence Color-Tuning Firefly Luciferases: Engineering and Prospects for Real-Time Intracellular pH Imaging and Heavy Metal Biosensing.Biosensors · 2022Review
- Portable FRET-Based Biosensor Device for On-Site Lead Detection.Biosensors · 2022Article
- Fluorescent Indicators For Biological Imaging of Monatomic Ions.Frontiers in cell and developmental biology · 2022Review
- Article
- Development and optimization of heavy metal lead biosensors in biomedical and environmental applications.Journal of the Chinese Medical Association : JCMA · 2021Review
- Switching between Ultrafast Pathways Enables a Green-Red Emission Ratiometric Fluorescent-Protein-Based CaInternational journal of molecular sciences · 2021Article
- Combinatorial roles of mitochondria and cGMP/PKG pathway in the generation of neuronal free Zn2+ under the presence of nitric oxide.Journal of the Chinese Medical Association : JCMA · 2020Article
- Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular CdBiometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2019Article
- Engineering genetically encoded FRET-based nanosensors for real time display of arsenic (AsScientific reports · 2019Article
- A Ratiometric Fluorescent Sensor for CdSensors (Basel, Switzerland) · 2017Article
- Fluorescent sensors for measuring metal ions in living systems.Chemical reviews · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cd(2+) causes damages to several human tissues. Although the toxicological and carcinogenetic mechanisms of Cd(2+) have been previously established, some basic questions on this toxicant remain unclear. In this study, we constructed Met-cad 1.57, a new fluorescent resonance energy transfer (FRET)-based Cd(2+) indicator, which contains a portion of a Cd(2+)-binding protein (CadR) obtained from Pseudomonas putida as the Cd(2+) sensing key. We produced a human embryonic kidney cell line HEK-MCD157 which stably expresses the Met-cad 1.57 for further investigations. Both fluorescence spectroscopy and FRET microscopic ratio imaging were used to monitor the Cd(2+) concentration within the living HEK-MCD157 cells. The dissociation constant of Met-cad 1.57 was approximately 271 nM. The function of Ca(2+) channels as a potential Cd(2+) entry gateway was further confirmed in the HEK-MCD157 cells. The organelle-targeted property of the protein-based Cd(2+) indicator directly reveals the nucleus accumulation phenomena. In summary, a human kidney cell line that stably expresses the FRET-based Cd(2+) indicator Met-cad 1.57 was constructed for reliable and convenient investigations to determine the Cd(2+) concentration within living cells, including the identification of the entry pathway of Cd(2+) and sub-cellular sequestration.
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