ArticlePloS one2013
Diversity of plant methionine sulfoxide reductases B and evolution of a form specific for free methionine sulfoxide.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular timekeepers: the curious alliance of redox, repair, and protective proteins in preserving seed longevity.Crop health · 2026Review
- Genome-Wide Identification and Characterization of the Msr Gene Family in Alfalfa under Abiotic Stress.International journal of molecular sciences · 2023Article
- Function of the evolutionarily conserved plant methionine-S-sulfoxide reductase without the catalytic residue.Protoplasma · 2018Article
- Methionine Sulfoxide Reductases of Archaea.Antioxidants (Basel, Switzerland) · 2018Review
- Physiological Roles of Plant Methionine Sulfoxide Reductases in Redox Homeostasis and Signaling.Antioxidants (Basel, Switzerland) · 2018Review
- Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics.International journal of molecular sciences · 2016Review
- Practical guide for dynamic monitoring of protein oxidation using genetically encoded ratiometric fluorescent biosensors of methionine sulfoxide.Methods (San Diego, Calif.) · 2016Review
- The Impact of Non-Enzymatic Reactions and Enzyme Promiscuity on Cellular Metabolism during (Oxidative) Stress Conditions.Biomolecules · 2015Review
- Protein Methionine Sulfoxide Dynamics in Arabidopsis thaliana under Oxidative Stress.Molecular & cellular proteomics : MCP · 2015Article
- A pepper MSRB2 gene confers drought tolerance in rice through the protection of chloroplast-targeted genes.PloS one · 2014Article
- Differential expression analysis of a subset of drought-responsive GmNAC genes in two soybean cultivars differing in drought tolerance.International journal of molecular sciences · 2013Article
- Cloning, expression, and characterization of a methionine sulfoxide reductase B gene from Nicotiana tabacum.The protein journal · 2013Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Methionine can be reversibly oxidized to methionine sulfoxide (MetO) under physiological conditions. Organisms evolved two distinct methionine sulfoxide reductase families (MSRA & MSRB) to repair oxidized methionine residues. We found that 5 MSRB genes exist in the soybean genome, including GmMSRB1 and two segmentally duplicated gene pairs (GmMSRB2 and GmMSRB5, GmMSRB3 and GmMSRB4). GmMSRB2 and GmMSRB4 proteins showed MSRB activity toward protein-based MetO with either DTT or thioredoxin (TRX) as reductants, whereas GmMSRB1 was active only with DTT. GmMSRB2 had a typical MSRB mechanism with Cys121 and Cys 68 as catalytic and resolving residues, respectively. Surprisingly, this enzyme also possessed the MSRB activity toward free Met-R-O with kinetic parameters similar to those reported for fRMSR from Escherichia coli, an enzyme specific for free Met-R-O. Overexpression of GmMSRB2 or GmMSRB4 in the yeast cytosol supported the growth of the triple MSRA/MSRB/fRMSR (Δ3MSRs) mutant on MetO and protected cells against H2O2-induced stress. Taken together, our data reveal an unexpected diversity of MSRBs in plants and indicate that, in contrast to mammals that cannot reduce free Met-R-O and microorganisms that use fRMSR for this purpose, plants evolved MSRBs for the reduction of both free and protein-based MetO.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.