ArticleProceedings of the National Academy of Sciences of the United States of America2013
Reversion of a fungal genetic code alteration links proteome instability with genomic and phenotypic diversification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Review
- The transcriptome of dendritic cells redraws the boundaries between pathogenicity and commensalism in yeast.Microbial cell (Graz, Austria) · 2026Article
- Predicting the Structural Effects of CUG Codon Translation on Uncharacterized Proteins inJournal of fungi (Basel, Switzerland) · 2025Article
- D-stem mutation in an essential tRNA increases translation speed at the cost of fidelity.PLoS genetics · 2025Article
- Genomics insights of candidiasis: mechanisms of pathogenicity and drug resistance.Frontiers in microbiology · 2025Review
- Histone-like transcription factor Hfl1p inVirulence · 2024Article
- tRNA regulation and amino acid usage bias reflect a coordinated metabolic adaptation iniScience · 2024Article
- Ncs2* mediates in vivo virulence of pathogenic yeast through sulphur modification of cytoplasmic transfer RNA.Nucleic acids research · 2023Article
- Post-transcriptional control of antifungal resistance in human fungal pathogens.Critical reviews in microbiology · 2023Review
- The tRNA identity landscape for aminoacylation and beyond.Nucleic acids research · 2023Review
- Increase in Ribosomal Fidelity BenefitsGenes · 2022Article
- Proteogenomics analysis of CUG codon translation in the human pathogen Candida albicans.BMC biology · 2021Article
- The role of non-standard translation in Candida albicans pathogenesis.FEMS yeast research · 2021Review
- The impact of mistranslation on phenotypic variability and fitness.Evolution; international journal of organic evolution · 2021Article
- Analysis of Stop Codons within Prokaryotic Protein-Coding Genes Suggests Frequent Readthrough Events.International journal of molecular sciences · 2021Article
- tRNAs as a Driving Force of Genome Evolution in Yeast.Frontiers in microbiology · 2021Review
- An Overview on Conventional and Non-Conventional Therapeutic Approaches for the Treatment of Candidiasis and Underlying Resistance Mechanisms in Clinical Strains.Journal of fungi (Basel, Switzerland) · 2020Review
- Human cells adapt to translational errors by modulating protein synthesis rate and protein turnover.RNA biology · 2020Article
- Pathways to disease from natural variations in human cytoplasmic tRNAs.The Journal of biological chemistry · 2019Review
- PHO15 genes of Candida albicans and Candida parapsilosis encode HAD-type phosphatases dephosphorylating 2-phosphoglycolate.FEMS yeast research · 2019Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many fungi restructured their proteomes through incorporation of serine (Ser) at thousands of protein sites coded by the leucine (Leu) CUG codon. How these fungi survived this potentially lethal genetic code alteration and its relevance for their biology are not understood. Interestingly, the human pathogen Candida albicans maintains variable Ser and Leu incorporation levels at CUG sites, suggesting that this atypical codon assignment flexibility provided an effective mechanism to alter the genetic code. To test this hypothesis, we have engineered C. albicans strains to misincorporate increasing levels of Leu at protein CUG sites. Tolerance to the misincorporations was very high, and one strain accommodated the complete reversion of CUG identity from Ser back to Leu. Increasing levels of Leu misincorporation decreased growth rate, but production of phenotypic diversity on a phenotypic array probing various metabolic networks, drug resistance, and host immune cell responses was impressive. Genome resequencing revealed an increasing number of genotype changes at polymorphic sites compared with the control strain, and 80% of Leu misincorporation resulted in complete loss of heterozygosity in a large region of chromosome V. The data unveil unanticipated links between gene translational fidelity, proteome instability and variability, genome diversification, and adaptive phenotypic diversity. They also explain the high heterozygosity of the C. albicans genome and open the door to produce microorganisms with genetic code alterations for basic and applied research.
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