ArticleProceedings of the National Academy of Sciences of the United States of America2013
General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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Who cites it
46 citing papers in PubMed, 117 citations in OpenAlex.
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- Substrate-Specific Differences in Catalytic Strategy and Activity across Ketol-Acid Reductoisomerase Variants.The journal of physical chemistry. B · 2026Article
- Monooxygenase-dehydrogenase cascade for sustained enzymatic remediation of TMA in salmon protein hydrolysates.Applied and environmental microbiology · 2026Article
- Nitrogen starvation induces arbuscular mycorrhizal fungi to optimize resource allocation in sugarcane roots via suppression of basal metabolism.NPJ biofilms and microbiomes · 2026Article
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- Engineering the carbon and redox metabolism of Paenibacillus polymyxa for efficient isobutanol production.Microbial biotechnology · 2024Article
- Pseudomonas putida as a platform for medium-chain length α,ω-diol production: Opportunities and challenges.Microbial biotechnology · 2024Article
- Exploring the "misfolding problem" by systematic discovery and analysis of functional-but-degraded proteins.Molecular biology of the cell · 2023Article
- Optimal energy and redox metabolism in the cyanobacterium Synechocystis sp. PCC 6803.NPJ systems biology and applications · 2023Article
- Pathogenic bacteria remodel central metabolic enzyme to build a cyclopropanol warhead.Nature chemistry · 2022Article
- Engineering Isopropanol Dehydrogenase for Efficient Regeneration of Nicotinamide Cofactors.Applied and environmental microbiology · 2022Article
- Characterization of a class II ketol-acid reductoisomerase fromRSC advances · 2022Article
- Rossmann-toolbox: a deep learning-based protocol for the prediction and design of cofactor specificity in Rossmann fold proteins.Briefings in bioinformatics · 2022Article
- Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production.Nature communications · 2022Article
- Combinatorial library design for improving isobutanol production inFrontiers in bioengineering and biotechnology · 2022Article
- Structural Rearrangements of a Dodecameric Ketol-Acid Reductoisomerase Isolated from a Marine Thermophilic Methanogen.Biomolecules · 2021Article
- Functional Analysis of Keto-Acid Reductoisomerase ILVC in the Entomopathogenic FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Isobutanol Production by Autotrophic Acetogenic Bacteria.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
To date, efforts to switch the cofactor specificity of oxidoreductases from nicotinamide adenine dinucleotide phosphate (NADPH) to nicotinamide adenine dinucleotide (NADH) have been made on a case-by-case basis with varying degrees of success. Here we present a straightforward recipe for altering the cofactor specificity of a class of NADPH-dependent oxidoreductases, the ketol-acid reductoisomerases (KARIs). Combining previous results for an engineered NADH-dependent variant of Escherichia coli KARI with available KARI crystal structures and a comprehensive KARI-sequence alignment, we identified key cofactor specificity determinants and used this information to construct five KARIs with reversed cofactor preference. Additional directed evolution generated two enzymes having NADH-dependent catalytic efficiencies that are greater than the wild-type enzymes with NADPH. High-resolution structures of a wild-type/variant pair reveal the molecular basis of the cofactor switch.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.