ArticleProceedings of the National Academy of Sciences of the United States of America2013
Phosphorylation of the Bin, Amphiphysin, and RSV161/167 (BAR) domain of ACAP4 regulates membrane tubulation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- ASAP3 activates the NF-κB signaling pathway to promote the progression of esophageal squamous cell carcinoma.Discover oncology · 2026Article
- BAR-PH tandem of ACAP4 remodels membranes to drive migration via Ezrin-dependent activation.Journal of molecular cell biology · 2026Article
- Dimerization of ACAP4 is essential for its function in cell migration.Journal of molecular cell biology · 2025Article
- Akt-elicited phosphorylation of Acapin steers cell migration.Journal of molecular cell biology · 2025Article
- Kupffer Cell-derived IL6 Promotes Hepatocellular Carcinoma Metastasis Via the JAK1-ACAP4 Pathway.International journal of biological sciences · 2025Article
- The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients.Open medicine (Warsaw, Poland) · 2022Article
- Lysine acetylation regulates the interaction between proteins and membranes.Nature communications · 2021Article
- Measurement of dynamic membrane mechanosensation using optical tweezers.Journal of molecular cell biology · 2021Article
- An Abl-FBP17 mechanosensing system couples local plasma membrane curvature and stress fiber remodeling during mechanoadaptation.Nature communications · 2019Article
- Acetylation of ACAP4 regulates CCL18-elicited breast cancer cell migration and invasion.Journal of molecular cell biology · 2018Article
- DDEFL1 correlated with Rho GTPases activity in breast cancer.Oncotarget · 2017Article
- MST4 kinase phosphorylates ACAP4 protein to orchestrate apical membrane remodeling during gastric acid secretion.The Journal of biological chemistry · 2017Article
- Upregulation of ASAP3 contributes to colorectal carcinogenesis and indicates poor survival outcome.Cancer science · 2017Article
- IRE1-RACK1 axis orchestrates ER stress preconditioning-elicited cytoprotection from ischemia/reperfusion injury in liver.Journal of molecular cell biology · 2016Article
- Dependence of cardiac transverse tubules on the BAR domain protein amphiphysin II (BIN-1).Circulation research · 2014Article
- ASAP3 regulates microvilli structure in parietal cells and presents intervention target for gastric acidity.Signal transduction and targeted therapyArticle
Corrections and comments
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Authors and funding
19 authors at 7 institutions in 2 countries.
Funding
Abstract
ArfGAP With Coiled-Coil, Ankyrin Repeat And PH Domains 4 (ACAP4) is an ADP-ribosylation factor 6 (ARF6) GTPase-activating protein essential for EGF-elicited cell migration. However, how ACAP4 regulates membrane dynamics and curvature in response to EGF stimulation is unknown. Here, we show that phosphorylation of the N-terminal region of ACAP4, named the Bin, Amphiphysin, and RSV161/167 (BAR) domain, at Tyr34 is necessary for EGF-elicited membrane remodeling. Domain structure analysis demonstrates that the BAR domain regulates membrane curvature. EGF stimulation of cells causes phosphorylation of ACAP4 at Tyr34, which subsequently promotes ACAP4 homodimer curvature. The phospho-mimicking mutant of ACAP4 demonstrates lipid-binding activity and tubulation in vitro, and ARF6 enrichment at the membrane is associated with ruffles of EGF-stimulated cells. Expression of the phospho-mimicking ACAP4 mutant promotes ARF6-dependent cell migration. Thus, the results present a previously undefined mechanism by which EGF-elicited phosphorylation of the BAR domain controls ACAP4 molecular plasticity and plasma membrane dynamics during cell migration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.