ArticleJournal of lipid research2013
Analysis of inflammatory and lipid metabolic networks across RAW264.7 and thioglycolate-elicited macrophages.
Article in Journal of lipid research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
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- Oxysterols in Vascular Cells and Role in Atherosclerosis.Advances in experimental medicine and biology · 2024Article
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- Immunolipidomics Reveals a Globoside Network During the Resolution of Pro-Inflammatory Response in Human Macrophages.Frontiers in immunology · 2022Article
- ATF3 Positively Regulates Antibacterial Immunity by Modulating Macrophage Killing and Migration Functions.Frontiers in immunology · 2022Article
- Anti-inflammatory effect of gold nanoparticles supported on metal oxides.Scientific reports · 2021Article
- Abrogation of LRRK2 dependent Rab10 phosphorylation with TLR4 activation and alterations in evoked cytokine release in immune cells.Neurochemistry international · 2021Article
- Dual Effect of Soloxolone Methyl on LPS-Induced Inflammation In Vitro and In Vivo.International journal of molecular sciences · 2020Article
- Cholesterol 25-hydroxylase promotes efferocytosis and resolution of lung inflammation.JCI insight · 2020Article
- Multi-omics Analysis of Liver Infiltrating Macrophages Following Ethanol Consumption.Scientific reports · 2019Article
- Gamma-tocotrienol attenuates the aberrant lipid mediator production in NLRP3 inflammasome-stimulated macrophages.The Journal of nutritional biochemistry · 2018Article
- Altered eicosanoid production and phospholipid remodeling during cell culture.Journal of lipid research · 2018Article
- An Integrated Proteomics and Bioinformatics Approach Reveals the Anti-inflammatory Mechanism of Carnosic Acid.Frontiers in pharmacology · 2018Article
- Krüppel-Like Factor 4 Regulation of Cholesterol-25-Hydroxylase and Liver X Receptor Mitigates Atherosclerosis Susceptibility.Circulation · 2017Article
- Impact of phenolic compounds in the acyl homoserine lactone-mediated quorum sensing regulatory pathways.Scientific reports · 2017Article
- Antioxidant and Anti-Inflammatory Effects of Herbal Formula SC-E3 in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.Evidence-based complementary and alternative medicine : eCAM · 2017Article
- Computational Modeling of Competitive Metabolism between ω3- and ω6-Polyunsaturated Fatty Acids in Inflammatory Macrophages.The journal of physical chemistry. B · 2016Article
Corrections and comments
- Erratum issuedERRATA.2014
Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
Studies of macrophage biology have been significantly advanced by the availability of cell lines such as RAW264.7 cells. However, it is unclear how these cell lines differ from primary macrophages such as thioglycolate-elicited peritoneal macrophages (TGEMs). We used the inflammatory stimulus Kdo2-lipid A (KLA) to stimulate RAW264.7 and TGEM cells. Temporal changes of lipid and gene expression levels were concomitantly measured and a systems-level analysis was performed on the fold-change data. Here we present a comprehensive comparison between the two cell types. Upon KLA treatment, both RAW264.7 and TGEM cells show a strong inflammatory response. TGEM (primary) cells show a more rapid and intense inflammatory response relative to RAW264.7 cells. DNA levels (fold-change relative to control) are reduced in RAW264.7 cells, correlating with greater downregulation of cell cycle genes. The transcriptional response suggests that the cholesterol de novo synthesis increases considerably in RAW264.7 cells, but 25-hydroxycholesterol increases considerably in TGEM cells. Overall, while RAW264.7 cells behave similarly to TGEM cells in some ways and can be used as a good model for inflammation- and immune function-related kinetic studies, they behave differently than TGEM cells in other aspects of lipid metabolism and phenotypes used as models for various disorders such as atherosclerosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.