ArticleNucleic acids research2013
Rapid oligonucleotide-templated fluorogenic tetrazine ligations.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Enhanced through-bond energy transfer-based bioorthogonal probe enables catalytic-amplified and sensitive detection of microRNA-21 for clinical lung cancer diagnosis.Smart molecules : open access · 2026Article
- Chemical autoligation with phosphorothioate- and sulfonamide-terminated DNA via intramolecular cross-activation.Communications chemistry · 2025Article
- Double Click: Unexpected 1:2 Stoichiometry in a Norbornene-Tetrazine Reaction.The Journal of organic chemistry · 2023Article
- Immune-responsive biodegradable scaffolds for enhancing neutrophil regeneration.Bioengineering & translational medicine · 2023Article
- Live-Cell Imaging of Sterculic Acid-a Naturally Occurring 1,2-Cyclopropene Fatty Acid-by Bioorthogonal Reaction with Turn-On Tetrazine-Fluorophore Conjugates.Angewandte Chemie (International ed. in English) · 2022Article
- [4 + 2] and [2 + 4] cycloaddition reactions on single- and double-stranded DNA: a dual-reactive nucleoside.RSC chemical biology · 2022Article
- Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing.ACS chemical biology · 2022Article
- Hydrolysis of 5-methylfuran-2-yl to 2,5-dioxopentanyl allows for stable bio-orthogonal proximity-induced ligation.Communications chemistry · 2021Article
- Covalent labeling of nucleic acids.Chemical Society reviews · 2020Review
- Labelling of DNA and RNA in the cellular environment by means of bioorthogonal cycloaddition chemistry.RSC chemical biology · 2020Review
- A Toxic RNA Templates the Synthesis of Its Own Fluorogenic Inhibitor by Using a Bio-orthogonal Tetrazine Ligation in Cells and Tissues.ACS chemical biology · 2020Article
- Templated chemistry for bioorganic synthesis and chemical biology.Journal of peptide science : an official publication of the European Peptide Society · 2019Review
- cat-ELCCA: catalyzing drug discovery through click chemistry.Chemical communications (Cambridge, England) · 2018Article
- Recyclable heterogeneous metal foil-catalyzed cyclopropenation of alkynes and diazoacetates under solvent-free mechanochemical reaction conditions.Chemical science · 2018Article
- Advances in Tetrazine Bioorthogonal Chemistry Driven by the Synthesis of Novel Tetrazines and Dienophiles.Accounts of chemical research · 2018Article
- Vinyluridine as a Versatile Chemoselective Handle for the Post-transcriptional Chemical Functionalization of RNA.Bioconjugate chemistry · 2017Article
- Subnanomolar Detection of Oligonucleotides through Templated Fluorogenic Reaction in Hydrogels: Controlling Diffusion to Improve Sensitivity.Angewandte Chemie (International ed. in English) · 2017Article
- Modern Synthetic Avenues for the Preparation of Functional Fluorophores.Angewandte Chemie (International ed. in English) · 2017Review
- Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels-Alder Reactions.Chembiochem : a European journal of chemical biology · 2017Review
- Responsive Hybrid (Poly)peptide-Polymer Conjugates.Journal of materials chemistry. B · 2017Article
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3 authors.
Funding
Abstract
Template driven chemical ligation of fluorogenic probes represents a powerful method for DNA and RNA detection and imaging. Unfortunately, previous techniques have been hampered by requiring chemistry with sluggish kinetics and background side reactions. We have developed fluorescent DNA probes containing quenched fluorophore-tetrazine and methyl-cyclopropene groups that rapidly react by bioorthogonal cycloaddition in the presence of complementary DNA or RNA templates. Ligation increases fluorescence with negligible background signal in the absence of hybridization template. Reaction kinetics depend heavily on template length and linker structure. Using this technique, we demonstrate rapid discrimination between single template mismatches both in buffer and cell media. Fluorogenic bioorthogonal ligations offer a promising route towards the fast and robust fluorescent detection of specific DNA or RNA sequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.