ArticleJournal of medical virology2013
Rotavirus shedding in symptomatic and asymptomatic children using reverse transcription-quantitative PCR.
Article in Journal of medical virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 2 syntheses or guidelines pooled it, 68 citations in OpenAlex.
- Systematic review and meta-analysis of enteric virus shedding in human excretions.EBioMedicine · 2025Pooled it
- Systematic Review on the Correlation of Quantitative PCR Cycle Threshold Values of Gastrointestinal Pathogens With Patient Clinical Presentation and Outcomes.Frontiers in medicine · 2021Pooled it
- Differences in Rotavirus Shedding and Duration by Infant Oral Rotavirus Vaccination Status in Dhaka, Bangladesh, 2011-2014.The Journal of infectious diseases · 2024Trial
- Efficacy of a monovalent human-bovine (116E) rotavirus vaccine in Indian infants: a randomised, double-blind, placebo-controlled trial.Lancet (London, England) · 2014Trial
- Kinetics of Enteric Pathogen Quantity During Acute Diarrhea in Children in Resource-Limited Settings.Open forum infectious diseases · 2026Article
- Mapping the Literature on the Impact of Gastrointestinal Multiplexed Pathogen Panels on Clinical and Healthcare Utilization Outcomes: A Scoping Review.The journal of applied laboratory medicine · 2026Article
- Importance of a heat snap in RT-PCR quantification of rotavirus double-stranded RNA in wastewater.Environmental science : water research & technology · 2025Article
- Wastewater concentrations of rotavirus RNA are associated with infection and vaccination metrics in the USA.Npj viruses · 2025Article
- Burden of rotavirus and other microorganisms in hospitalized children with acute gastroenteritis in Yangon, Myanmar, before the introduction of rotavirus vaccine.IJID regions · 2025Article
- Comparative Insights into Acute Gastroenteritis in Cattle Caused by Bovine Rotavirus A and Bovine Coronavirus.Veterinary sciences · 2024Review
- Temporal and spatial relationships of CrAssphage and enteric viral and bacterial pathogens in wastewater in North Carolina.Water research · 2023Article
- Article
- Global public health implications of human exposure to viral contaminated water.Frontiers in microbiology · 2022Review
- Norovirus and Other Viral Causes of Medically Attended Acute Gastroenteritis Across the Age Spectrum: Results from the Medically Attended Acute Gastroenteritis Study in the United States.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Duration of Postdiarrheal Enteric Pathogen Carriage in Young Children in Low-resource Settings.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Community transmission of rotavirus infection in a vaccinated population in Blantyre, Malawi: a prospective household cohort study.The Lancet. Infectious diseases · 2021Article
- Duration and Density of Fecal Rotavirus Shedding in Vaccinated Malawian Children With Rotavirus Gastroenteritis.The Journal of infectious diseases · 2020Article
- Occurrence, fates and potential treatment approaches for removal of viruses from wastewater: A review with emphasis on SARS-CoV-2.Journal of environmental chemical engineering · 2020Review
- Histo-blood group antigens and rotavirus vaccine shedding in Nicaraguan infants.Scientific reports · 2019Article
- Children Attending Day Care Centers are a Year-round Reservoir of Gastrointestinal Viruses.Scientific reports · 2019Observational
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Reverse transcription-real-time polymerase chain reaction (RT-qPCR) for the VP6 gene was used to study group A rotavirus shedding in children with symptomatic and asymptomatic rotavirus infection. Sequential stool samples (n = 345) from 10 children with rotavirus associated diarrhea and from five children (n = 161) with asymptomatic rotavirus infection were collected over a period of 2 months. A RT-qPCR assay on the samples using a rotavirus VP6 plasmid standard demonstrated high reproducibility, with an inter-assay coefficient of variation (CV) of 1.40-2.97% and an intra-assay CV of 0.03-3.03%. The median duration of shedding was longer in children with diarrhea compared to asymptomatic children (24 days vs. 18 days; P = 0.066). The median quantitation cycle (C(q)) at presentation in symptomatic children was 17.21 compared to 30.98 in asymptomatic children (P = 0.086). The temporal pattern in symptomatic children consisted of a high initial viral shedding coinciding with the duration of diarrhea, followed by a rapid fall, and then a small increase in secondary shedding 21 days later. Compared to children with rotavirus diarrhea, those with asymptomatic infection shed lower quantities of virus throughout the observation period. No difference was noted between the G and P genotypes of samples collected at onset of infection and during the shedding period. Shedding was intermittent in a subset of children in both groups. RT-qPCR is a useful method to characterize shedding patterns.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.