ArticleThe Journal of biological chemistry2013
A crayfish insulin-like-binding protein: another piece in the androgenic gland insulin-like hormone puzzle is revealed.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Effects of RNA Interference-Mediated Silencing of the Insulin-Like Androgenic Gland Hormone Gene on Growth and Gonad Development in the Swimming Crab (Animals : an open access journal from MDPI · 2026Article
- Life stage-dependent RNA interference in the tropical rock lobsterMarine life science & technology · 2026Article
- Uncovering the Eyestalk-Androgenic Gland-Testis Regulatory Axis Underlying Male Sexual Development of the Giant Freshwater Prawn Macrobrachium Rosenbergii: Insights from Multi-Tissue Comparative Transcriptomics Analysis.Marine biotechnology (New York, N.Y.) · 2026Article
- The Proteinase PAPP-A has Deep Evolutionary Roots Outside of the IGF System.Genome biology and evolution · 2025Article
- A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense.Scientific reports · 2024Article
- The effects ofJournal of ginseng research · 2024Article
- Transcriptomic Analysis across Crayfish (International journal of molecular sciences · 2024Article
- Identification of a Putative CFSH Receptor Inhibiting IAG Expression in Crabs.International journal of molecular sciences · 2023Article
- Article
- Transcriptomic Changes Following Induced De-Masculinisation of Australian Red Claw CrayfishInternational journal of molecular sciences · 2023Article
- Insulin-like growth factor signalling and its significance as a biomarker in fish and shellfish research.Fish physiology and biochemistry · 2021Review
- Insulin-Like Peptide Receptor-Mediated Signaling Pathways Orchestrate Regulation of Growth in the Pacific Oyster (International journal of molecular sciences · 2021Article
- The Chinese mitten crab genome provides insights into adaptive plasticity and developmental regulation.Nature communications · 2021Article
- Review
- Characterization and Expression Analysis of Insulin Growth Factor Binding Proteins (IGFBPs) in Pacific White ShrimpInternational journal of molecular sciences · 2021Article
- Article
- Characterization of Insulin-Like Growth Factor Binding Protein 7 (Igfbp7) and Its Potential Involvement in Shell Formation and Metamorphosis of Pacific Abalone,International journal of molecular sciences · 2020Article
- Article
- Insight into the Regulatory Relationships between the Insulin-Like Androgenic Gland Hormone Gene and the Insulin-Like Androgenic Gland Hormone-binding Protein Gene in Giant Freshwater Prawns (International journal of molecular sciences · 2020Article
- Molecular Characterization of the Insulin-Like Androgenic Gland Hormone in the Swimming Crab,Frontiers in endocrinology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Across the animal kingdom, the involvement of insulin-like peptide (ILP) signaling in sex-related differentiation processes is attracting increasing attention. Recently, a gender-specific ILP was identified as the androgenic sex hormone in Crustacea. However, moieties modulating the actions of this androgenic insulin-like growth factor were yet to be revealed. Through molecular screening of an androgenic gland (AG) cDNA library prepared from the crayfish Cherax quadricarinatus, we have identified a novel insulin-like growth factor-binding protein (IGFBP) termed Cq-IGFBP. Based on bioinformatics analyses, the deduced Cq-IGFBP was shown to share high sequence homology with IGFBP family members from both invertebrates and vertebrates. The protein also includes a sequence determinant proven crucial for ligand binding, which according to three-dimensional modeling is assigned to the exposed outer surface of the protein. Recombinant Cq-IGFBP (rCq-IGFBP) protein was produced and, using a "pulldown" methodology, was shown to specifically interact with the insulin-like AG hormone of the crayfish (Cq-IAG). Particularly, using both mass spectral analysis and an immunological tool, rCq-IGFBP was shown to bind the Cq-IAG prohormone. Furthermore, a peptide corresponding to residues 23-38 of the Cq-IAG A-chain was found sufficient for in vitro recognition by rCq-IGFBP. Cq-IGFBP is the first IGFBP family member shown to specifically interact with a gender-specific ILP. Unlike their ILP ligands, IGFBPs are highly conserved across evolution, from ancient arthropods, like crustaceans, to humans. Such conservation places ILP signaling at the center of sex-related phenomena in early animal development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.