ArticleThe Journal of biological chemistry2013
Generating S-nitrosothiols from hemoglobin: mechanisms, conformational dependence, and physiological relevance.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Biosynthetic blood surrogates: Current status and future opportunities.Bioengineering & translational medicine · 2025Review
- The chemical biology of dinitrogen trioxide.Redox biochemistry and chemistry · 2024Article
- NO and Heme Proteins: Cross-Talk between Heme and Cysteine Residues.Antioxidants (Basel, Switzerland) · 2023Review
- Nitrite in breast milk: roles in neonatal pathophysiology.Pediatric research · 2021Review
- βCysteine 93 in human hemoglobin: a gateway to oxidative stability in health and disease.Laboratory investigation; a journal of technical methods and pathology · 2021Review
- Role of Nitric Oxide Carried by Hemoglobin in Cardiovascular Physiology: Developments on a Three-Gas Respiratory Cycle.Circulation research · 2020Review
- Hemoglobin-based Oxygen Carriers: Current State-of-the-art and Novel Molecules.Shock (Augusta, Ga.) · 2019Review
- Erythrocytic bioactivation of nitrite and its potentiation by far-red light.Redox biology · 2019Article
- Differential mitochondrial dinitrosyliron complex formation by nitrite and nitric oxide.Redox biology · 2018Article
- Erythrocytes and Vascular Function: Oxygen and Nitric Oxide.Frontiers in physiology · 2018Review
- Bio-inspired nanomedicine strategies for artificial blood components.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2017Review
- HbE/β-Thalassemia and Oxidative Stress: The Key to Pathophysiological Mechanisms and Novel Therapeutics.Antioxidants & redox signaling · 2017Review
- Recent insights into nitrite signaling processes in blood.Biological chemistry · 2017Article
- A mathematical model for the role of NNitric oxide : biology and chemistry · 2016Article
- The role of red blood cell S-nitrosation in nitrite bioactivation and its modulation by leucine and glucose.Redox biology · 2016Article
- Molecular Properties of Globin Channels and Pores: Role of Cholesterol in Ligand Binding and Movement.Frontiers in physiology · 2016Article
- Evaluating the capacity to generate and preserve nitric oxide bioactivity in highly purified earthworm erythrocruorin: a giant polymeric hemoglobin with potential blood substitute properties.The Journal of biological chemistry · 2015Article
- Blood substitutes: why haven't we been more successful?Trends in biotechnology · 2014Review
- The emerging roles of somatic globins in cardiovascular redox biology and beyond.Redox biology · 2013Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In vitro, ferrous deoxy-hemes in hemoglobin (Hb) react with nitrite to generate nitric oxide (NO) through a nitrite reductase reaction. In vivo studies indicate Hb with nitrite can be a source of NO bioactivity. The nitrite reductase reaction does not appear to account fully for this activity because free NO is short lived especially within the red blood cell. Thus, the exporting of NO bioactivity both out of the RBC and over a large distance requires an additional mechanism. A nitrite anhydrase (NA) reaction in which N2O3, a potent S-nitrosating agent, is produced through the reaction of NO with ferric heme-bound nitrite has been proposed (Basu, S., Grubina, R., Huang, J., Conradie, J., Huang, Z., Jeffers, A., Jiang, A., He, X., Azarov, I., Seibert, R., Mehta, A., Patel, R., King, S. B., Hogg, N., Ghosh, A., Gladwin, M. T., and Kim-Shapiro, D. B. (2007) Nat. Chem. Biol. 3, 785-794) as a possible mechanism. Legitimate concerns, including physiological relevance and the nature of the mechanism, have been raised concerning the NA reaction. This study addresses these concerns demonstrating NO and nitrite with ferric hemes under near physiological conditions yield an intermediate having the properties of the purported NA heme-bound N2O3 intermediate. The results indicate that ferric heme sites, traditionally viewed as a source of potential toxicity, can be functionally significant, especially for partially oxygenated/partially met-R state Hb that arises from the NO dioxygenation reaction. In the presence of low levels of nitrite and either NO or a suitable reductant such as L-cysteine, these ferric heme sites can function as a generator for the formation of S-nitrosothiols such as S-nitrosoglutathione and, as such, should be considered as a source of RBC-derived and exportable bioactive NO.
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Registered trials
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