ArticleInfection and immunity2013
Production and release of antimicrobial and immune defense proteins by mammary epithelial cells following Streptococcus uberis infection of sheep.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Milk proteins as mastitis markers in dairy ruminants - a systematic review.Veterinary research communications · 2022Pooled it
- Appraisal of the Use of Proteomics Methodological Approaches and Technologies on Sheep and Goat Research and Clinical Work.Animals : an open access journal from MDPI · 2025Article
- Detection of Cathelicidin-1 and Cathelicidin-2 Biomolecules in the Milk of Goats and Their Use as Biomarkers for the Diagnosis of Mastitis.Animals : an open access journal from MDPI · 2025Article
- Impact of a teat disinfectant based on Lactococcus cremoris on the cow milk proteome.BMC veterinary research · 2024Article
- Biomarkers for subclinical bovine mastitis: a high throughput TMT-based proteomic investigation.Veterinary research communications · 2024Article
- Evaluation of ARFI elastography for detecting active mastitis in sheep with previous fibrous lesions: a study of mammary parenchyma and supramammary lymph nodes.Animal reproduction · 2024Article
- Comprehensive proteomic analysis reveals omega-3 fatty acids to counteract endotoxin-stimulated metabolic dysregulation in porcine enterocytes.Scientific reports · 2023Article
- Unique Peptides of Cathelicidin-1 in the Early Detection of Mastitis-In Silico Analysis.International journal of molecular sciences · 2023Article
- Immune defenses of the mammary gland epithelium of dairy ruminants.Frontiers in immunology · 2022Review
- Potential Novel Biomarkers for Mastitis Diagnosis in Sheep.Animals : an open access journal from MDPI · 2021Review
- Applied Proteomics in 'One Health'.Proteomes · 2021Review
- Expression Pattern of Cathelicidins in Dairy Cows During Endometritis and Role of Bovine Endometrial Epithelial Cells in Production of Cathelicidins.Frontiers in veterinary science · 2021Article
- A Paradox in Bacterial Pathogenesis: Activation of the Local Macrophage Inflammasome Is Required for Virulence ofPathogens (Basel, Switzerland) · 2020Article
- Cathelicidins Mitigate Staphylococcus aureus Mastitis and Reduce Bacterial Invasion in Murine Mammary Epithelium.Infection and immunity · 2020Article
- Relationship of Late Lactation Milk Somatic Cell Count and Cathelicidin with Intramammary Infection in Small Ruminants.Pathogens (Basel, Switzerland) · 2020Article
- Murine and Human Cathelicidins Contribute Differently to Hallmarks of Mastitis Induced by PathogenicFrontiers in cellular and infection microbiology · 2020Article
- Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes.Pathogens (Basel, Switzerland) · 2019Article
- Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci.Scientific reports · 2019Article
- Mammary Defences and Immunity against Mastitis in Sheep.Animals : an open access journal from MDPI · 2019Review
- Use of Proteomics in the Study of Mastitis in Ewes.Pathogens (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Investigating the innate immune response mediators released in milk has manifold implications, spanning from elucidation of the role played by mammary epithelial cells (MECs) in fighting microbial infections to the discovery of novel diagnostic markers for monitoring udder health in dairy animals. Here, we investigated the mammary gland response following a two-step experimental infection of lactating sheep with the mastitis-associated bacterium Streptococcus uberis. The establishment of infection was confirmed both clinically and by molecular methods, including PCR and fluorescent in situ hybridization of mammary tissues. Proteomic investigation of the milk fat globule (MFG), a complex vesicle released by lactating MECs, enabled detection of enrichment of several proteins involved in inflammation, chemotaxis of immune cells, and antimicrobial defense, including cathelicidins and calprotectin (S100A8/S100A9), in infected animals, suggesting the consistent involvement of MECs in the innate immune response to pathogens. The ability of MECs to produce and release antimicrobial and immune defense proteins was then demonstrated by immunohistochemistry and confocal immunomicroscopy of cathelicidin and the calprotectin subunit S100A9 on mammary tissues. The time course of their release in milk was also assessed by Western immunoblotting along the course of the experimental infection, revealing the rapid increase of these proteins in the MFG fraction in response to the presence of bacteria. Our results support an active role of MECs in the innate immune response of the mammary gland and provide new potential for the development of novel and more sensitive tools for monitoring mastitis in dairy animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.