ArticleInfection and immunity2013
Genetic analysis of the role of yfiR in the ability of Escherichia coli CFT073 to control cellular cyclic dimeric GMP levels and to persist in the urinary tract.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Genetic requirements for uropathogenicmSystems · 2024Article
- CsrA-Mediated Translational Activation of theJournal of bacteriology · 2023Article
- YfiB: An Outer Membrane Protein Involved in the Virulence ofMicroorganisms · 2022Article
- The enhanced expression of genes encoding diguanylate cyclases under cold stress contributes to the adhesion and biofilm formation ofFrontiers in nutrition · 2022Article
- A Cyclic di-GMP Network Is Present in Gram-PositiveACS infectious diseases · 2020Article
- An Osmoregulatory Mechanism Operating through OmpR and LrhA Controls the Motile-Sessile Switch in the Plant Growth-Promoting BacteriumApplied and environmental microbiology · 2019Article
- Article
- A bipartite periplasmic receptor-diguanylate cyclase pair (XAC2383-XAC2382) in the bacteriumThe Journal of biological chemistry · 2018Article
- The Catabolite Repressor/Activator Cra Is a Bridge Connecting Carbon Metabolism and Host Colonization in the Plant Drought Resistance-Promoting Bacterium Pantoea alhagi LTYR-11Z.Applied and environmental microbiology · 2018Article
- Cyclic-di-GMP regulation of virulence in bacterial pathogens.Wiley interdisciplinary reviews. RNA · 2018Review
- Temperature-Dependent Gene Expression inFrontiers in microbiology · 2018Article
- Altered Regulation of the Diguanylate Cyclase YaiC Reduces Production of Type 1 Fimbriae in a Pst Mutant of Uropathogenic Escherichia coli CFT073.Journal of bacteriology · 2017Article
- Article
- Structural insights into the regulatory mechanism of the Pseudomonas aeruginosa YfiBNR system.Protein & cell · 2016Article
- Systematic Nomenclature for GGDEF and EAL Domain-Containing Cyclic Di-GMP Turnover Proteins of Escherichia coli.Journal of bacteriology · 2016Article
- Structural insights into YfiR sequestering by YfiB in Pseudomonas aeruginosa PAO1.Scientific reports · 2015Article
- Adherent-Invasive Escherichia coli Production of Cellulose Influences Iron-Induced Bacterial Aggregation, Phagocytosis, and Induction of Colitis.Infection and immunity · 2015Article
- YjjQ Represses Transcription of flhDC and Additional Loci in Escherichia coli.Journal of bacteriology · 2015Article
- Positive autoregulation of mrkHI by the cyclic di-GMP-dependent MrkH protein in the biofilm regulatory circuit of Klebsiella pneumoniae.Journal of bacteriology · 2015Article
- The disulfide bonding system suppresses CsgD-independent cellulose production in Escherichia coli.Journal of bacteriology · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
During urinary tract infections (UTIs), uropathogenic Escherichia coli must maintain a delicate balance between sessility and motility to achieve successful infection of both the bladder and kidneys. Previous studies showed that cyclic dimeric GMP (c-di-GMP) levels aid in the control of the transition between motile and nonmotile states in E. coli. The yfiRNB locus in E. coli CFT073 contains genes for YfiN, a diguanylate cyclase, and its activity regulators, YfiR and YfiB. Deletion of yfiR yielded a mutant that was attenuated in both the bladder and the kidneys when tested in competition with the wild-type strain in the murine model of UTI. A double yfiRN mutant was not attenuated in the mouse model, suggesting that unregulated YfiN activity and likely increased cytoplasmic c-di-GMP levels cause a survival defect. Curli fimbriae and cellulose production were increased in the yfiR mutant. Expression of yhjH, a gene encoding a proven phosphodiesterase, in CFT073 ΔyfiR suppressed the overproduction of curli fimbriae and cellulose and further verified that deletion of yfiR results in c-di-GMP accumulation. Additional deletion of csgD and bcsA, genes necessary for curli fimbriae and cellulose production, respectively, returned colonization levels of the yfiR deletion mutant to wild-type levels. Peroxide sensitivity assays and iron acquisition assays displayed no significant differences between the yfiR mutant and the wild-type strain. These results indicate that dysregulation of c-di-GMP production results in pleiotropic effects that disable E. coli in the urinary tract and implicate the c-di-GMP regulatory system as an important factor in the persistence of uropathogenic E. coli in vivo.
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