Evidence map›Paper›PMID 23773572›Full record

ArticleGenome biology2013

User guide for mapping-by-sequencing in Arabidopsis.

Geo Velikkakam James, Vipul Patel, Karl J V Nordström, Jonas R Klasen, Patrice A Salomé, Detlef Weigel, Korbinian Schneeberger

Open access · goldAbstract read
In one paragraph

Article in Genome biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
18.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 142 citations in OpenAlex.

  1. Article
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  5. Review
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  9. Article
  10. Versatile mapping-by-sequencing with Easymap v.2.Frontiers in plant science · 2023
    Article
  11. Review
  12. Article
  13. Article
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  18. Review
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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Geo Velikkakam James
Vipul Patel
Karl J V Nordström
Jonas R Klasen
Patrice A Salomé
Detlef Weigel
Korbinian Schneeberger
Max Planck Institute for Developmental Biology · DEMax Planck Institute for Plant Breeding Research · DEMax Planck Society · DESaarland University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mapping-by-sequencing combines genetic mapping with whole-genome sequencing in order to accelerate mutant identification. However, application of mapping-by-sequencing requires decisions on various practical settings on the experimental design that are not intuitively answered. Following an experimentally determined recombination landscape of Arabidopsis and next generation sequencing-specific biases, we simulated more than 400,000 mapping-by-sequencing experiments. This allowed us to evaluate a broad range of different types of experiments and to develop general rules for mapping-by-sequencing in Arabidopsis. Most importantly, this informs about the properties of different crossing scenarios, the number of recombinants and sequencing depth needed for successful mapping experiments.

Indexed as

Chromosomes, PlantGenome, PlantArabidopsisBreedingChromosome MappingCrosses, GeneticHigh-Throughput Nucleotide SequencingMutationRecombination, Genetic

Identifiers

PMID23773572
PMCPMC3706810
OpenAlexW2021549775

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.