ArticleThe Analyst2013
Detection of mRNA in living cells by double-stranded locked nucleic acid probes.
Article in The Analyst, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Bone-derived nanoparticles (BNPs) enhance osteogenic differentiationNanoscale advances · 2025Article
- Involvement of long non-coding RNA (lncRNA) MALAT1 in shear stress regulated adipocyte differentiation.Frontiers in bioengineering and biotechnology · 2025Article
- Long noncoding RNA MALAT1 is dynamically regulated in leader cells during collective cancer invasion.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nrf2 Modulates the Hybrid Epithelial/Mesenchymal Phenotype and Notch Signaling During Collective Cancer Migration.Frontiers in molecular biosciences · 2022Article
- Colour compound lenses for a portable fluorescence microscope.Light, science & applications · 2019Article
- Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes.RSC advances · 2018Article
- A gapmer aptamer nanobiosensor for real-time monitoring of transcription and translation in single cells.Biomaterials · 2018Article
- A nanobiosensor for dynamic single cell analysis during microvascular self-organization.Nanoscale · 2016Article
- Probing 3D Collective Cancer Invasion Using Double-Stranded Locked Nucleic Acid Biosensors.Analytical chemistry · 2016Article
- Notch1-Dll4 signalling and mechanical force regulate leader cell formation during collective cell migration.Nature communications · 2015Article
- Article
- Oncogenic KRAS confers chemoresistance by upregulating NRF2.Cancer research · 2014Article
- Mapping photothermally induced gene expression in living cells and tissues by nanorod-locked nucleic acid complexes.ACS nano · 2014Article
- Single cell gene expression analysis in injury-induced collective cell migration.Integrative biology : quantitative biosciences from nano to macro · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Double-stranded probes are homogeneous biosensors for rapid detection of specific nucleotide sequences. These double-stranded probes have been applied in various molecular sensing applications, such as real-time polymerase chain reaction and detection of bacterial 16S rRNA. In this study, we present the design and optimization of double-stranded probes for single-cell gene expression analysis in living cells. With alternating DNA/LNA monomers for optimizing the stability and specificity, we show that the probe is stable in living cells for over 72 hours post-transfection and is capable of detecting changes in gene expression induced by external stimuli. The probes can be delivered to a large number of cells simultaneously by cationic liposomal transfection or to individual cells selectively by photothermal delivery. We also demonstrate that the probe quantifies intracellular mRNA in living cells through the use of an equilibrium analysis. With its effectiveness and performance, the double-stranded probe represents a broadly applicable approach for large-scale single-cell gene expression analysis toward numerous biomedical applications, such as systems biology, cancer, and drug screening.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.