ArticleJournal of bacteriology2013
Isolation and characterization of mutant Sinorhizobium meliloti NodD1 proteins with altered responses to luteolin.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Regulation of Rhizobial Nodulation Genes by Flavonoid-Independent NodD Supports Nitrogen-Fixing Symbioses With Legumes.Environmental microbiology · 2025Article
- Calcium Induces the Cleavage of NopA and Regulates the Expression of Nodulation Genes and Secretion of T3SS Effectors inInternational journal of molecular sciences · 2024Article
- A complex regulatory network governs the expression of symbiotic genes inFrontiers in plant science · 2023Article
- The Rhizobium tropici CIAT 899 NodD2 protein promotes symbiosis and extends rhizobial nodulation range by constitutive nodulation factor synthesis.Journal of experimental botany · 2022Article
- The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium-Legume Symbiosis.International journal of molecular sciences · 2022Review
- Surface Motility Regulation ofInternational journal of molecular sciences · 2022Article
- PsrA is a novel regulator contributes to antibiotic synthesis, bacterial virulence, cell motility and extracellular polysaccharides production in Serratia marcescens.Nucleic acids research · 2022Article
- OnfD, an AraC-Type Transcriptional Regulator Encoded byApplied and environmental microbiology · 2020Article
- Symbiosis islands of Loteae-nodulatingMicrobial genomics · 2020Article
- The Rhizobium tropici CIAT 899 NodD2 protein regulates the production of Nod factors under salt stress in a flavonoid-independent manner.Scientific reports · 2017Article
- Structural basis for regulation of rhizobial nodulation and symbiosis gene expression by the regulatory protein NolR.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bradyrhizobium elkanii nod regulon: insights through genomic analysis.Genetics and molecular biologyArticle
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
NodD1, a member of the NodD family of LysR-type transcriptional regulators (LTTRs), mediates nodulation (nod) gene expression in the soil bacterium Sinorhizobium meliloti in response to the plant-secreted flavonoid luteolin. We used genetic screens and targeted approaches to identify NodD1 residues that show altered responses to luteolin during the activation of nod gene transcription. Here we report four types of NodD1 mutants. Type I (NodD1 L69F, S104L, D134N, and M193I mutants) displays reduced or no activation of nod gene expression. Type II (NodD1 K205N) is constitutively active but repressed by luteolin. Type III (NodD1 L280F) demonstrates enhanced activity with luteolin compared to that of wild-type NodD1. Type IV (NodD1 D284N) shows moderate constitutive activity yet can still be induced by luteolin. In the absence of luteolin, many mutants display a low binding affinity for nod gene promoter DNA in vitro. Several mutants also show, as does wild-type NodD1, increased affinity for nod gene promoters with added luteolin. All of the NodD1 mutant proteins can homodimerize and heterodimerize with wild-type NodD1. Based on these data and the crystal structures of several LTTRs, we present a structural model of wild-type NodD1, identifying residues important for inducer binding, protein multimerization, and interaction with RNA polymerase at nod gene promoters.
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