ArticleJournal of bacteriology2013
Genetic and physiological responses of Bifidobacterium animalis subsp. lactis to hydrogen peroxide stress.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Microbiome-host communication via vaccenic acid modulates LRH-1/NR5A2 activity and ameliorates metabolic liver disease.EMBO molecular medicine · 2026Article
- Bifidobacterium lactis ameliorates AOM/DSS-induced inflammation, dysbiosis, and colonic precancerous lesions.Applied microbiology and biotechnology · 2025Article
- Taxonomic Identification of the Arctic StrainInternational journal of molecular sciences · 2023Article
- Understanding the Probiotic Bacterial Responses Against Various Stresses in Food Matrix and Gastrointestinal Tract: A Review.Probiotics and antimicrobial proteins · 2023Review
- Novel Insights into the Molecular Mechanisms Underlying Robustness and Stability in Probiotic Bifidobacteria.Applied and environmental microbiology · 2023Article
- Stress Response in Bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2022Review
- Cysteine protected cells from HWorld journal of microbiology & biotechnology · 2022Article
- Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria.Applied and environmental microbiology · 2022Article
- Revisiting long-chain fatty acid metabolism in Escherichia coli: integration with stress responses.Current genetics · 2021Review
- Effects of Linoleic Acid on Gut-DerivedMicroorganisms · 2019Article
- Development of omics-based protocols for the microbiological characterization of multi-strain formulations marketed as probiotics: the case of VSL#3.Microbial biotechnology · 2019Article
- The complete genome sequence of3 Biotech · 2019Article
- Adaptational changes in physiological and transcriptional responses of Bifidobacterium longum involved in acid stress resistance after successive batch cultures.Microbial cell factories · 2019Article
- Transcriptomic analysis of Bifidobacterium longum subsp. longum BBMN68 in response to oxidative shock.Scientific reports · 2018Article
- Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria.Journal of industrial microbiology & biotechnology · 2015Article
- The influence of fatty acids on the GpA dimer interface by coarse-grained molecular dynamics simulation.International journal of molecular sciences · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Consumer interest in probiotic bifidobacteria is increasing, but industry efforts to secure high cell viability in foods is undermined by these anaerobes' sensitivity to oxidative stress. To address this limitation, we investigated genetic and physiological responses of two fully sequenced Bifidobacterium animalis subsp. lactis strains, BL-04 and DSM 10140, to hydrogen peroxide (H₂O₂) stress. Although the genome sequences for these strains are highly clonal, prior work showed that they differ in both intrinsic and inducible H₂O₂ resistance. Transcriptome analysis of early-stationary-phase cells exposed to a sublethal H₂O₂ concentration detected significant (P < 0.05) changes in expression of 138 genes in strain BL-04 after 5 min and 27 genes after 20 min. Surprisingly, no significant changes in gene expression were detected in DSM 10140 at either time. Genomic data suggested that differences in H₂O₂ stress resistance might be due to a mutation in a BL-04 gene encoding long-chain fatty acid coenzyme A (CoA) ligase. To explore this possibility, membrane fatty acids were isolated and analyzed by gas chromatography-mass spectrometry (GC-MS). Results confirmed that the strains had significantly different lipid profiles: the BL-04 membrane contained higher percentages of C(14:0) and C(16:0) and lower percentages of C(18:1n9). Alteration of the DSM 10140 membrane lipid composition using modified growth medium to more closely mimic that of BL-04 yielded cells that showed increased intrinsic resistance to lethal H₂O₂ challenge but did not display an inducible H₂O₂ stress response. The results show that deliberate stress induction or membrane lipid modification can be employed to significantly improve H₂O₂ resistance in B. animalis subsp. lactis strains.
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