Evidence map›Paper›PMID 23771591›Full record

ArticleJournal of biomolecular NMR2013

Divide and conquer is always best: sensitivity of methyl correlation experiments.

Kaustubh Sinha, Linda Jen-Jacobson, Gordon S Rule

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Biomolecular NMR assignments · 2020
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kaustubh SinhaDepartment of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Linda Jen-Jacobson
Gordon S Rule

Funding

Sequence Specific DNA Protein InteractionsR37GM029207 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI JEN-JACOBSON, LINDA · 2001 to 2010
$4.1M
SEQUENCE-SPECIFIC DNA-PROTEIN INTERACTIONSR01GM029207 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI JEN-JACOBSON, LINDA · 1985 to 2018
$1.6M
NIGMS NIH HHS 5R37-GM029207NIGMS NIH HHS R01 GM029207NIGMS NIH HHS R37 GM029207
6 · The paper itself

Abstract

The HMCM [CG]CBCA experiment (Tugarinov and Kay in J Am Chem Soc 125:13868-13878, 2003) correlates methyl carbon and proton shifts to Cγ, Cβ, and Cα resonances for the purpose of resonance assignments. The relative sensitivity of the HMCM[CG]CBCA sequence experiment is compared to a divide-and-conquer approach to assess whether it is best to collect all of the methyl correlations at once, or to perform separate experiments for each correlation. A straightforward analysis shows that the divide-and-conquer approach is intrinsically more sensitive, and should always be used to obtain methyl-Cγ, Cβ, and Cα correlations. The improvement in signal-to-noise associated with separate experiments is illustrated by the detection of methyl-aliphatic correlations in a 65 kDa protein-DNA complex.

Indexed as

Nuclear Magnetic Resonance, BiomolecularDeoxyribonucleases, Type II Site-SpecificDNAEscherichia coliEscherichia coli ProteinsLeucineMethylationDeoxyribonucleases, Type II Site-SpecificDNAEscherichia coli ProteinsGATATC-specific type II deoxyribonucleasesLeucine

Identifiers

PMID23771591
PMCPMC3758368

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.