ArticleScientific reports2013
Automated parallel recordings of topologically identified single ion channels.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 146 citations in OpenAlex.
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- Droplet Contact Method (DCM) for Stable and Reproducible Lipid Bilayer Formation and its Application to Nanopore Measurements.Methods in molecular biology (Clifton, N.J.) · 2026Article
- De Novo Design of α-helical Peptide Nanopores for Single-Molecule Detection Using Helix Packing Motifs.ACS nano · 2025Article
- Intercalant Aggregation Promotes Nanoscopic Depletion in Droplet Interface Bilayers.The journal of physical chemistry. B · 2025Article
- Direct insertion of an ion channel immobilized on a soft agarose gel bead into a lipid bilayer: an optimized method.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Efficient single-channel current measurements of the human BK channel using a liposome-immobilized gold probe.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Electrophysiological Characterization of Monoolein-Fatty Acid Bilayers.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Cell-Penetrating Peptide-Mediated Biomolecule Transportation in Artificial Lipid Vesicles and Living Cells.Molecules (Basel, Switzerland) · 2024Review
- Activity of Single Insect Olfactory Receptors Triggered by Airborne Compounds Recorded in Self-Assembled Tethered Lipid Bilayer Nanoarchitectures.ACS applied materials & interfaces · 2023Article
- Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes.International journal of molecular sciences · 2023Review
- Cell-Free Expression ofACS nano · 2023Article
- Liposome Deformation Induced by Membrane-Binding Peptides.Micromachines · 2023Article
- Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies.Soft matter · 2022Article
- De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide.Nature nanotechnology · 2022Article
- Development of an automated system to measure ion channel currents using a surface-modified gold probe.Scientific reports · 2021Article
- Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules.Micromachines · 2021Article
- Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray.Micromachines · 2021Article
- Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence.Molecules (Basel, Switzerland) · 2021Article
- Efficient Lipid Bilayer Formation by Dipping Lipid-Loaded Microperforated Sheet in Aqueous Solution.Micromachines · 2021Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although ion channels are attractive targets for drug discovery, the systematic screening of ion channel-targeted drugs remains challenging. To facilitate automated single ion-channel recordings for the analysis of drug interactions with the intra- and extracellular domain, we have developed a parallel recording methodology using artificial cell membranes. The use of stable lipid bilayer formation in droplet chamber arrays facilitated automated, parallel, single-channel recording from reconstituted native and mutated ion channels. Using this system, several types of ion channels, including mutated forms, were characterised by determining the protein orientation. In addition, we provide evidence that both intra- and extracellular amyloid-beta fragments directly inhibit the channel open probability of the hBK channel. This automated methodology provides a high-throughput drug screening system for the targeting of ion channels and a data-intensive analysis technique for studying ion channel gating mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.