ArticleThe EMBO journal2013
Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 67 citations in OpenAlex.
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- Heterochromatin-based silencing of a foreign tandem repeat inbioRxiv : the preprint server for biology · 2025Article
- RiboCop surveils pre-rRNA processing by Dicer in cellular quiescence.bioRxiv : the preprint server for biology · 2025Article
- Mechanistic insights into the stimulation of the histone H3K9 methyltransferase Clr4 by proximal H3K14 ubiquitination.Science advances · 2025Article
- Fission yeast Caprin protein is required for efficient heterochromatin establishment.PLoS genetics · 2025Article
- H3K14: A histone site closely related to diseases.Journal of Cancer · 2025Review
- Heterochromatin repeat organization at an individual level: Rex1BD and the 14-3-3 protein coordinate to shape the epigenetic landscape within heterochromatin repeats.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.Cell · 2024Article
- Mathematical model for the role of multiple pericentromeric repeats on heterochromatin assembly.PLoS computational biology · 2024Article
- Acetylation State of Lysine 14 of Histone H3.3 Affects Mutant Huntingtin Induced Pathogenesis.International journal of molecular sciences · 2022Article
- Facultative heterochromatin formation in rDNA is essential for cell survival during nutritional starvation.Nucleic acids research · 2022Article
- SETDB1-like MET-2 promotes transcriptional silencing and development independently of its H3K9me-associated catalytic activity.Nature structural & molecular biology · 2022Article
- Breakers and amplifiers in chromatin circuitry: acetylation and ubiquitination control the heterochromatin machinery.Current opinion in structural biology · 2021Review
- Subtelomeric Chromatin in the Fission YeastMicroorganisms · 2021Review
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- Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements inmBio · 2021Article
- Surprising phenotypic diversity of cancer-associated mutations of Gly 34 in the histone H3 tail.eLife · 2021Article
- Close to the edge: Heterochromatin at the nucleolar and nuclear peripheries.Biochimica et biophysica acta. Gene regulatory mechanisms · 2021Review
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6 authors at 2 institutions in 1 country.
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Abstract
Heterochromatin assembly in fission yeast depends on the Clr4 histone methyltransferase, which targets H3K9. We show that the histone deacetylase Sir2 is required for Clr4 activity at telomeres, but acts redundantly with Clr3 histone deacetylase to maintain centromeric heterochromatin. However, Sir2 is critical for Clr4 function during de novo centromeric heterochromatin assembly. We identified new targets of Sir2 and tested if their deacetylation is necessary for Clr4-mediated heterochromatin establishment. Sir2 preferentially deacetylates H4K16Ac and H3K4Ac, but mutation of these residues to mimic acetylation did not prevent Clr4-mediated heterochromatin establishment. Sir2 also deacetylates H3K9Ac and H3K14Ac. Strains bearing H3K9 or H3K14 mutations exhibit heterochromatin defects. H3K9 mutation blocks Clr4 function, but why H3K14 mutation impacts heterochromatin was not known. Here, we demonstrate that recruitment of Clr4 to centromeres is blocked by mutation of H3K14. We suggest that Sir2 deacetylates H3K14 to target Clr4 to centromeres. Further, we demonstrate that Sir2 is critical for de novo accumulation of H3K9me2 in RNAi-deficient cells. These analyses place Sir2 and H3K14 deacetylation upstream of Clr4 recruitment during heterochromatin assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.