ArticleApplied and environmental microbiology2013
Biosynthesis of the osmoprotectant ectoine, but not glycine betaine, is critical for survival of osmotically stressed Vibrio parahaemolyticus cells.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 59 citations in OpenAlex.
- Deciphering the evolutionary history of ectoine catabolism, a compatible solute utilized byApplied and environmental microbiology · 2026Article
- Genomic and phenotypic characterization of Vibrio cholerae strain SU109: a genomic investigation of a shrimp-associated strain from Bangladesh.BMC genomics · 2026Article
- Article
- Dual roles of glycine betaine, dimethylglycine, and sarcosine as osmoprotectants and nutrient sources forApplied and environmental microbiology · 2025Article
- Destructive and protective effects and therapeutic targets of IL-36 family cytokines in dry eye disease.The ocular surface · 2025Article
- Draft genome sequence of the marine halophileMicrobiology resource announcements · 2025Article
- Vibrio natriegens: Application of a Fast-Growing Halophilic Bacterium.Advances in biochemical engineering/biotechnology · 2025Review
- Functional and Genomic Insights into the Biotechnological Potential of Vibrio spp. Isolated from Deeply Polluted and Pristine Environments.Current microbiology · 2024Article
- Significance of Viable But Non-culturable (VBNC) State in Vibrios and Other Pathogenic Bacteria: Induction, Detection and the Role of Resuscitation Promoting Factors (Rpf).Current microbiology · 2024Review
- Metabolic pathway engineering of high-salinity-induced overproduction of L-proline improves high-salinity stress tolerance of an ectoine-deficientApplied and environmental microbiology · 2024Article
- Osmotic stress response of the coral and oyster pathogenApplied and environmental microbiology · 2024Article
- Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model.Investigative ophthalmology & visual science · 2024Article
- Ectoine, from a Natural Bacteria Protectant to a New Treatment of Dry Eye Disease.Pharmaceutics · 2024Article
- NhaR, LeuO, and H-NS Are Part of an Expanded Regulatory Network for Ectoine Biosynthesis Expression.Applied and environmental microbiology · 2023Article
- Vibrio natriegens genome-scale modeling reveals insights into halophilic adaptations and resource allocation.Molecular systems biology · 2023Article
- Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival.Advances in experimental medicine and biology · 2023Article
- Examining the Osmotic Response ofMicroorganisms · 2021Article
- Accumulation of Ectoines By Halophilic Bacteria Isolated from Fermented Shrimp Paste: An Adaptation Mechanism to Salinity, Temperature, and pH Stress.Current microbiology · 2021Article
- Phenotypic and genomic characterization of aMicrobial genomics · 2021Article
- The organosulfur compound dimethylsulfoniopropionate (DMSP) is utilized as an osmoprotectant byApplied and environmental microbiology · 2021Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Vibrio parahaemolyticus is a halophile present in marine and estuarine environments, ecosystems characterized by fluctuations in salinity and temperature. One strategy to thrive in such environments is the synthesis and/or uptake of compatible solutes. The V. parahaemolyticus genome contains biosynthesis systems for both ectoine and glycine betaine, which are known to act as compatible solutes in other species. We showed that V. parahaemolyticus had a 6% NaCl tolerance when grown in M9 minimal medium with 0.4% glucose (M9G) with a >5-h lag phase. By using (1)H nuclear magnetic resonance spectroscopy ((1)H-NMR) analysis, we determined that cells synthesized ectoine and glutamate in a NaCl-dependent manner. The most effective compatible solutes as measured by a reduction in lag-phase growth in M9G with 6% NaCl (M9G 6% NaCl) were in the order glycine betaine > choline > proline = glutamate > ectoine. However, V. parahaemolyticus could use glutamate or proline as the sole carbon source, but not ectoine or glycine betaine, which suggests that these are bona fide compatible solutes. Expression analysis showed that the ectA and betA genes were more highly expressed in log-phase cells, and expression of both genes was induced by NaCl up-shock. Under all conditions examined, the ectA gene was more highly expressed than the betA gene. Analysis of in-frame deletions in betA and ectB and in a double mutant showed that the ectB mutant was defective for growth, and this defect was rescued by the addition of glycine betaine, proline, ectoine, and glutamate, indicating that these compounds are compatible solutes for this species. The presence of both synthesis systems was the predominant distribution pattern among members of the Vibrionaceae family, suggesting this is the ancestral state.
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