ArticleNature structural & molecular biology2013
Bimodal expression of PHO84 is modulated by early termination of antisense transcription.
Article in Nature structural & molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed.
- A systematic interactome ofeLife · 2026Article
- Transcriptional interference revisited.Nature genetics · 2026Review
- Functional analysis and transcriptional profiling of non-coding RNAs in yeast.The Biochemical journal · 2025Review
- Transcriptional heterogeneity shapes stress-adaptive responses in yeast.Nature communications · 2025Article
- RNA exosome-driven RNA processing instructs the duration of the unfolded protein response.Nucleic acids research · 2025Article
- Identification and functional analysis of growth rate associated long non-coding RNAs inComputational and structural biotechnology journal · 2025Article
- Multilevel Regulation of Membrane Proteins in Response to Metal and Metalloid Stress: A Lesson from Yeast.International journal of molecular sciences · 2024Review
- The genomic region of the 3' untranslated region (3'UTR) of PHO84, rather than the antisense RNA, promotes gene repression.Nucleic acids research · 2023Article
- The Nrd1-Nab3-Sen1 transcription termination complex from a structural perspective.Biochemical Society transactions · 2023Review
- Diverse and dynamic forms of gene regulation by the S. cerevisiae histone methyltransferase Set1.Current genetics · 2023Review
- Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure.PLoS genetics · 2022Article
- Modulated termination of non-coding transcription partakes in the regulation of gene expression.Nucleic acids research · 2022Article
- Phosphate Restriction Promotes Longevity via Activation of Autophagy and the Multivesicular Body Pathway.Cells · 2021Article
- Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.Cell reports · 2021Article
- Yeast RNA exosome activity is necessary for maintaining cell wall stability through proper protein glycosylation.Molecular biology of the cell · 2021Article
- An Overview on Identification and Regulatory Mechanisms of Long Non-coding RNAs in Fungi.Frontiers in microbiology · 2021Review
- Variation, Variegation and Heritable Gene Repression inFrontiers in genetics · 2021Review
- UNAGI: an automated pipeline for nanopore full-length cDNA sequencing uncovers novel transcripts and isoforms in yeast.Functional & integrative genomics · 2020Article
- The cryptic unstable transcripts are associated with developmentally regulated gene expression in blood-stageRNA biology · 2020Article
- Sense-antisense miRNA pairs constitute an elaborate reciprocal regulatory circuit.Genome research · 2020Article
33 more citing papers are in PubMed but not listed here.
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6 authors.
Funding
Abstract
Many Saccharomyces cerevisiae genes encode antisense transcripts, some of which are unstable and degraded by the exosome component Rrp6. Loss of Rrp6 results in the accumulation of long PHO84 antisense (AS) RNAs and repression of sense transcription through PHO84 promoter deacetylation. We used single-molecule resolution fluorescent in situ hybridization (smFISH) to investigate antisense-mediated transcription regulation. We show that PHO84 AS RNA acts as a bimodal switch, in which continuous, low-frequency antisense transcription represses sense expression within individual cells. Surprisingly, antisense RNAs do not accumulate at the PHO84 gene but are exported to the cytoplasm. Furthermore, rather than stabilizing PHO84 AS RNA, the loss of Rrp6 favors its elongation by reducing early transcription termination by Nrd1-Nab3-Sen1. These observations suggest that PHO84 silencing results from antisense transcription through the promoter rather than the static accumulation of antisense RNAs at the repressed gene.
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