Evidence map›Paper›PMID 23770363›Full record

ArticleBiochemical and biophysical research communications2013

Vitamin D upregulates glutamate cysteine ligase and glutathione reductase, and GSH formation, and decreases ROS and MCP-1 and IL-8 secretion in high-glucose exposed U937 monocytes.

Sushil K Jain, David Micinski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
113citing papers in PubMed, 4 pooled it
9.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

113 citing papers in PubMed, 4 syntheses or guidelines pooled it, 234 citations in OpenAlex.

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  11. N-Acetylcysteine rapidly replenishes central nervous system glutathione measured via magnetic resonance spectroscopy in human neonates with hypoxic-ischemic encephalopathy.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018
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53 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Sushil K JainDepartment of Pediatrics, Louisiana State University Health Sciences Center, Shreveport, LA 71130, United States. sjain@lsuhsc.edu
David Micinski
Louisiana State University Health Sciences Center Shreveport · US

Funding

Ketosis, Vascular Inflammation and Its Therapy in Type 1 Diabetic PatientsR01DK072433 · NIDDK · LOUISIANA STATE UNIV HSC SHREVEPORT · PI JAIN, SUSHIL K · 2007 to 2010
$1.3M
NIDDK NIH HHS R01 DK072433
6 · The paper itself

Abstract

introductionGlutathione is a major endogenous antioxidant and its deficiency is implicated in the etiology and progression of a number of human diseases. Vitamin D is important for the prevention of osteoporosis, cardiovascular disease, diabetes, autoimmune diseases, and some cancers. Using a monocyte cell model, this study examined the hypothesis that vitamin D upregulate glutamate cysteine ligase (GCLC) and glutathione reductase (GR), which catalyzes GSH biosynthesis.

methodsU937 monocytes were pretreated with and without 1,25 (OH)₂ vitamin D (10-25 nM) for 24 h and then exposed to control and high glucose (HG, 25 mM) for 4h. Levels of GSH determined using HPLC; GR activity by oxidation of NADPH; GCLC protein, MCP-1 and IL-8 using ELISA kits.

results1,25 (OH)₂ vitamin D supplementation significantly upregulated expression of GCLC and GR, levels of GCLC protein and GR activity, and formation of GSH in control and HG-treated monocytes. 1,25 (OH)₂ vitamin D caused significantly (p<0.05) lower secretion of IL-8 and MCP-1, and lower ROS levels in monocytes exposed to control and HG-treated monocytes.

conclusionsThis study demonstrates a positive link between vitamin D and GSH levels, and that some beneficial effects of vitamin D supplementation may be mediated by an improvement in the cellular GSH levels and a decrease in ROS and pro-inflammatory cytokines.

Indexed as

Chemokine CCL2GlucoseGlutamate-Cysteine LigaseGlutathioneGlutathione ReductaseHumansInterleukin-8MonocytesReactive Oxygen SpeciesU937 CellsUp-RegulationVitamin D1,25-dihydroxyvitamin DCCL2 protein, humanChemokine CCL2CXCL8 protein, humanGlucoseGlutamate-Cysteine LigaseGlutathioneGlutathione ReductaseInterleukin-8Reactive Oxygen SpeciesVitamin DDiabetesGCLCGCLMGlutamate cysteine ligaseglutamate cysteine ligase catalytic unitglutamate cysteine ligase modulatory unitglutathioneglutathione reductaseGlutathione reductaseGRGSHreactive oxygen speciesROSVitamin D

Identifiers

PMID23770363
PMCPMC4063448
OpenAlexW2018665788

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.