ArticleCurrent biology : CB2013
Transient and specific inactivation of Drosophila neurons in vivo using a native ligand-gated ion channel.
Article in Current biology : CB, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Motor neurons organize Drosophila feeding sequences via a disinhibitory cascade.Nature neuroscience · 2026Article
- Conflicting adaptations in an inhibitory feedback circuit.The Journal of physiology · 2026Article
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- Walking strides direct rapid and flexible recruitment of visual circuits for course control in Drosophila.Neuron · 2022Article
- A Quantitative Model of Sporadic Axonal Degeneration in theThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- A reversibly gated protein-transporting membrane channel made of DNA.Nature communications · 2022Article
- Localized inhibition in theeLife · 2020Article
- Mechanisms underlying homeostatic plasticity in theProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Recurrent Circuitry Sustains Drosophila Courtship Drive While Priming Itself for Satiety.Current biology : CB · 2019Article
- Co-option of a motor-to-sensory histaminergic circuit correlates with insect flight biomechanics.Proceedings. Biological sciences · 2017Article
- Article
- A Population of Projection Neurons that Inhibits the Lateral Horn but Excites the Antennal Lobe through Chemical Synapses inFrontiers in neural circuits · 2017Article
- Serotonergic Modulation Differentially Targets Distinct Network Elements within the Antennal Lobe of Drosophila melanogaster.Scientific reports · 2016Article
- Pharyngeal pumping in Caenorhabditis elegans depends on tonic and phasic signaling from the nervous system.Scientific reports · 2016Article
- Thermosensory processing in the Drosophila brain.Nature · 2015Article
- Modification of Male Courtship Motivation by Olfactory Habituation via the GABAA Receptor in Drosophila melanogaster.PloS one · 2015Article
- FMRFamide-like FLP-13 neuropeptides promote quiescence following heat stress in Caenorhabditis elegans.Current biology : CB · 2014Article
- Inducible and titratable silencing of Caenorhabditis elegans neurons in vivo with histamine-gated chloride channels.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A key tool in neuroscience is the ability to transiently inactivate specific neurons on timescales of milliseconds to minutes. In Drosophila, there are two available techniques for accomplishing this (shibire(ts) and halorhodopsin [1-3]), but both have shortcomings [4-9]. Here we describe a complementary technique using a native histamine-gated chloride channel (Ort). Ort is the receptor at the first synapse in the visual system. It forms large-conductance homomeric channels that desensitize only modestly in response to ligand [10]. Many regions of the CNS are devoid of histaminergic neurons [11, 12], raising the possibility that Ort could be used to artificially inactivate specific neurons in these regions. To test this idea, we performed in vivo whole-cell recordings from antennal lobe neurons misexpressing Ort. In these neurons, histamine produced a rapid and reversible drop in input resistance, clamping the membrane potential below spike threshold and virtually abolishing spontaneous and odor-evoked activity. Every neuron type in this brain region could be inactivated in this manner. Neurons that did not misexpress Ort showed negligible responses to histamine. Ort also performed favorably in comparison to the available alternative effector transgenes. Thus, Ort misexpression is a useful tool for probing functional connectivity among Drosophila neurons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.