Evidence map›Paper›PMID 23770009›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2013

Improving ultrasound gene transfection efficiency by controlling ultrasound excitation of microbubbles.

Z Fan, D Chen, C X Deng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
6.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 88 citations in OpenAlex.

  1. Article
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  3. Article
  4. Article
  5. Review
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  8. Review
  9. Applications of Ultrasound-Mediated Drug Delivery and Gene Therapy.International journal of molecular sciences · 2021
    Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Engineering patient-specific cancer immunotherapies.Nature biomedical engineering · 2019
    Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Z FanDepartment of Biomedical Engineering, University of Michigan, 2200 Bonisteel Boulevard, Ann Arbor, MI 48109, USA.
D Chen
C X Deng
University of Michigan–Ann Arbor · US

Funding

Mechanisms of ultrasound mediated intracellular drug and gene deliveryR01CA116592 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DENG, CHERI X · 2005 to 2009
$1.4M
NCI NIH HHS CA116592NCI NIH HHS R01 CA116592
6 · The paper itself

Abstract

Ultrasound application in the presence of microbubbles has shown great potential for non-viral gene transfection via transient disruption of cell membrane (sonoporation). However, improvement of its efficiency has largely relied on empirical approaches without consistent and translatable results. The goal of this study is to develop a rational strategy based on new results obtained using novel experimental techniques and analysis to improve sonoporation gene transfection. In this study, we conducted experiments using targeted microbubbles that were attached to cell membrane to facilitate sonoporation. We quantified the dynamic activities of microbubbles exposed to pulsed ultrasound and the resulting sonoporation outcome, and identified distinct regimes of characteristic microbubble behaviors: stable cavitation, coalescence and translation, and inertial cavitation. We found that inertial cavitation generated the highest rate of membrane poration. By establishing direct correlation of ultrasound-induced bubble activities with intracellular uptake and pore size, we designed a ramped pulse exposure scheme for optimizing microbubble excitation to improve sonoporation gene transfection. We implemented a novel sonoporation gene transfection system using an aqueous two phase system (ATPS) for efficient use of reagents and high throughput operation. Using plasmids coding for the green fluorescence protein (GFP), we achieved a sonoporation transfection efficiency in rate aortic smooth muscle cells (RASMCs) of 6.9%±2.2% (n=9), comparable with lipofection (7.5%±0.8%, n=9). Our results reveal characteristic microbubble behaviors responsible for sonoporation and demonstrated a rational strategy to improve sonoporation gene transfection.

Indexed as

MicrobubblesAcousticsAnimalsAortaCarbocyaninesCells, CulturedFluorescent DyesGreen Fluorescent ProteinsHumansHuman Umbilical Vein Endothelial CellsMyocytes, Smooth MusclePlasmidsRatsTransfectionBOBO 3CarbocyaninesFluorescent DyesGreen Fluorescent ProteinsGene transfectionHigh speed videomicroscopyIntracellular deliveryMicrobubblesSonoporationUltrasound

Identifiers

PMID23770009
PMCPMC3758692
OpenAlexW2023713300

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.