ArticleStructure (London, England : 1993)2013
Crystal structure of the 70S ribosome bound with the Q253P mutant form of release factor RF2.
Article in Structure (London, England : 1993), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- A cascade of structural rearrangements positions peptide release factor 2 for polypeptide hydrolysis on the ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome.Science (New York, N.Y.) · 2025Article
- Diversity and Similarity of Termination and Ribosome Rescue in Bacterial, Mitochondrial, and Cytoplasmic Translation.Biochemistry. Biokhimiia · 2021Review
- Ribosome Rescue Pathways in Bacteria.Frontiers in microbiology · 2021Review
- Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis.Nature communications · 2019Article
- Inhibition of translation termination by small molecules targeting ribosomal release factors.Scientific reports · 2019Article
- Article
- The structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy.Nature communications · 2019Article
- Translation in Prokaryotes.Cold Spring Harbor perspectives in biology · 2018Review
- Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World.Chemical reviews · 2018Review
- Mechanism of Inhibition of Translation Termination by Blasticidin S.Journal of molecular biology · 2018Article
- Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mechanism of ribosome rescue by ArfA and RF2.eLife · 2017Article
- Article
- Article
- Structural Basis for Translation Termination on a Pseudouridylated Stop Codon.Journal of molecular biology · 2016Article
- Peptide release promoted by methylated RF2 and ArfA in nonstop translation is achieved by an induced-fit mechanism.RNA (New York, N.Y.) · 2016Article
- Therapeutic suppression of premature termination codons: mechanisms and clinical considerations (review).International journal of molecular medicine · 2014Review
- Structure of the mammalian ribosomal pre-termination complex associated with eRF1.eRF3.GDPNP.Nucleic acids research · 2014Article
- RNA Bricks--a database of RNA 3D motifs and their interactions.Nucleic acids research · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial translation termination is mediated by release factors RF1 and RF2, which recognize stop codons and catalyze hydrolysis of the peptidyl-tRNA ester bond. The catalytic mechanism has been debated. We proposed that the backbone amide NH group, rather than the side chain, of the glutamine of the universally conserved GGQ motif participates in catalysis by H-bonding to the tetrahedral transition-state intermediate and by product stabilization. This was supported by complete loss of RF1 catalytic activity when glutamine is replaced by proline, the only residue that lacks a backbone NH group. Here, we present the 3.4 Å crystal structure of the ribosome complex containing the RF2 Q253P mutant and find that its fold, including the GGP sequence, is virtually identical to that of wild-type RF2. This rules out proline-induced misfolding and further supports the proposal that catalytic activity requires interaction of the Gln-253 backbone amide with the 3' end of peptidyl-tRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.