ArticleInvestigative ophthalmology & visual science2013
Doxycycline prevents matrix remodeling and contraction by trichiasis-derived conjunctival fibroblasts.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Oral doxycycline for the prevention of postoperative trachomatous trichiasis in Ethiopia: a randomised, double-blind, placebo-controlled trial.The Lancet. Global health · 2018Trial
- Eyelid Malpositions and Ocular Surface Disease: Clinical Correlations and Management Strategies.Journal of clinical medicine · 2025Review
- Effects of Losartan, Rapamycin, Doxycycline and botulinum toxin A: an experimentally induced urethral trauma model in rats.Journal of molecular histology · 2025Article
- Distinct Transcriptomic Profiles of Cultured Anterior and Posterior Populations of Human Infant Scleral Fibroblasts: Including Dopamine Receptors.Investigative ophthalmology & visual science · 2025Article
- Article
- The Emerging Role of MMP12 in the Oral Environment.International journal of molecular sciences · 2023Review
- Novel Anti-Inflammatory Approaches to COPD.International journal of chronic obstructive pulmonary disease · 2023Review
- Matrix Metalloproteinases and Glaucoma.Biomolecules · 2022Review
- The Anticancer Potential of Doxycycline and Minocycline-A Comparative Study on Amelanotic Melanoma Cell Lines.International journal of molecular sciences · 2022Article
- Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice.International journal of molecular sciences · 2021Article
- Effect of UVA1 on hypertrophic scarring in the rabbit ear model.Bioscience reports · 2020Article
- A Tenon's capsule/bulbar conjunctiva interface biomimetic to model fibrosis and local drug delivery.PloS one · 2020Article
- The Wound Healing Effect of Doxycycline after Corneal Alkali Burn in Rats.Journal of ophthalmology · 2019Article
- The conjunctival transcriptome in Ethiopians after trichiasis surgery: associations with the development of eyelid contour abnormalities and the effect of oral doxycycline treatment.Wellcome open research · 2019Article
- Genotype-Phenotype Associations of IL6 and PRG4 With Conjunctival Fibrosis After Glaucoma Surgery.JAMA ophthalmology · 2017Article
- Assessment of solid microneedle rollers to enhance transmembrane delivery of doxycycline and inhibition of MMP activity.Drug delivery · 2017Article
- Genome-wide RNA-Sequencing analysis identifies a distinct fibrosis gene signature in the conjunctiva after glaucoma surgery.Scientific reports · 2017Article
- An Ilomastat-CD Eye Drop Formulation to Treat Ocular Scarring.Investigative ophthalmology & visual science · 2017Article
- Local delivery of novel MRTF/SRF inhibitors prevents scar tissue formation in a preclinical model of fibrosis.Scientific reports · 2017Article
- Fibroblasts profiling in scarring trachoma identifies IL-6 as a functional component of a fibroblast-macrophage pro-fibrotic and pro-inflammatory feedback loop.Scientific reports · 2016Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
purposeTrachoma is a conjunctival scarring disease, which is the leading infectious cause of blindness worldwide. Elimination of blinding trachoma is being held back by the high rate of trichiasis recurrence following surgery. There is currently no treatment available to suppress the profibrotic state and reduce recurrence. Although the mechanisms underlying trichiasis development are unknown, the profibrotic phenotype has been linked to matrix metalloproteinase (MMP) expression. Doxycycline, a well-known tetracycline antibiotic, can act as a broad MMP inhibitor and has showed some success in preventing fibrosis in various clinical contexts. The purpose of this work was to assess the antiscarring properties of doxycycline in an in vitro model of trichiasis fibroblast-mediated tissue contraction.
methodsPrimary cultures of fibroblasts were established from conjunctival samples obtained from normal donors or during surgery for trachomatous trichiasis. The effect of doxycycline on matrix contraction was investigated in our standard collagen gel contraction model. Cell morphology and matrix integrity were assessed using confocal reflection microscopy. Quantitative real time polymerase chain reaction and a FRET-based assay were used to measure MMP expression and activity, respectively.
resultsDoxycycline treatment successfully suppressed the contractile phenotype of trichiasis fibroblasts, matrix degradation, and significantly altered the expression of MMP1, MMP9, and MMP12 associated with the profibrotic phenotype.
conclusionsIn view of the results presented here and the wider use of doxycycline in clinical settings, we propose that doxycycline might be useful as a treatment to prevent recurrence following trichiasis surgery.
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