ArticleThe British journal of ophthalmology2013
Pro-inflammatory and anti-angiogenic effects of bisphosphonates on human cultured retinal pigment epithelial cells.
Article in The British journal of ophthalmology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Zoledronic acid as adjuvant therapy in neovascular age-related macular degeneration: a randomised controlled pilot study.BMJ open ophthalmology · 2024Trial
- The effect of etidronate on choroidal neovascular activity in patients with pseudoxanthoma elasticum.PloS one · 2020Trial
- Effect of metformin on inflammation and bone damage in a rat model of medication-related osteonecrosis of the jaw.European journal of oral sciences · 2026Article
- Zoledronate enhances lipopolysaccharide-induced inflammation via toll-like receptor 4 upregulation.Journal of dental sciences · 2026Article
- Bisphosphonate-related ocular adverse events: a pharmacovigilance study based on the FAERS database.Endocrine connections · 2025Article
- Roles of growth factors in eye development and ophthalmic diseases.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022Review
- Pro-Calcific Environment Impairs Ischaemia-Driven Angiogenesis.International journal of molecular sciences · 2022Article
- Protein Microarrays for Ocular Diseases.Methods in molecular biology (Clifton, N.J.) · 2021Review
- A new anabolic compound, LLP2A-Ale, reserves periodontal bone loss in mice through augmentation of bone formation.BMC pharmacology & toxicology · 2020Article
- Risk of Cataract Surgery and Age-Related Macular Degeneration After Initiation of Denosumab vs Zoledronic Acid for Osteoporosis: A Multi-Database Cohort Study.Drugs & aging · 2020Article
- Alendronate induced chorioretinitis: The importance of meticulous assessments.American journal of ophthalmology case reports · 2019Article
- Oral bisphosphonate use and age-related macular degeneration: retrospective cohort and nested case-control study.Annals of the New York Academy of Sciences · 2018Article
- The role of angiogenesis in implant dentistry part I: Review of titanium alloys, surface characteristics and treatments.Medicina oral, patologia oral y cirugia bucal · 2016Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
aimBisphosphonates have been shown to induce ocular inflammatory diseases such as uveitis and scleritis, while being protective against angiogenic diseases like neovascular age-related macular degeneration (AMD). Therefore, we studied the effects of bisphosphonates on primary culture of human fetal retinal pigment epithelium (hRPE), a cell type known to secrete both inflammatory and angiogenic factors. Alendronate and etidronate were selected for this experiment as they are members of the two structurally different classes of bisphosphonates.
methodsPrimary cultures of hRPE were serum-starved for 24 h and then treated for 24 h with alendronate (0.0001, 0.1, 100 µM) or etidronate (0.01, 1 µM). Cell viability was measured using the MTT assay. Investigation of secreted cytokines induced by bisphosphonates was performed using a human cytokine 29-Plex Panel (Bio-Plex) array and the results were analysed with an analysis of variance (ANOVA).
resultsEtidronate, at the lower concentration, significantly increased the expression of interleukin (IL)-6 (p=0.03) and IL-8 (p=0.04). At the higher concentration, etidronate significantly decreased the expression of granulocyte macrophage colony-stimulating factor (p=0.02) and basic fibroblast growth factor (bFGF) (p=0.02). Alendronate, at the highest concentration, significantly increased the expression of IL-8 (p=0.02) and decreased the expression of eotaxin (p=0.02). Alendronate also significantly decreased the expression of bFGF at all concentrations (p<0.05) and demonstrated a trend towards decreasing vascular endothelial growth factor expression at low concentration.
conclusionsAlendronate and etidronate display dose dependent effects in hRPE cells. Alendronate and etidronate administration resulted in concentration dependent elevations in inflammatory cytokines. Furthermore, alendronate and etidronate administration resulted in reduced expression of a number of angiogenic factors. These findings may explain the increased incidence of ocular inflammation as well as the therapeutic effect on neovascular AMD which have been described with bisphosphonates.
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