ArticleNucleic acids research2013
Whole-body scanning PCR; a highly sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Peptide Conjugates of a 2'-ACS omega · 2023Article
- Future directions for medicinal chemistry in the field of oligonucleotide therapeutics.RNA (New York, N.Y.) · 2023Article
- Quantification of Antisense Oligonucleotides by Splint Ligation and Quantitative Polymerase Chain Reaction.Nucleic acid therapeutics · 2022Article
- Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Signatures of TSPAN8 variants associated with human metabolic regulation and diseases.iScience · 2021Article
- Delivery of oligonucleotides to bone marrow to modulate ferrochelatase splicing in a mouse model of erythropoietic protoporphyria.Nucleic acids research · 2020Article
- Quantitative PCR analysis of DNA aptamer pharmacokinetics in mice.Nucleic acid therapeutics · 2015Article
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Authors and funding
19 authors.
Funding
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Abstract
Efficient tissue-specific delivery is a crucial factor in the successful development of therapeutic oligonucleotides. Screening for novel delivery methods with unique tissue-homing properties requires a rapid, sensitive, flexible and unbiased technique able to visualize the in vivo biodistribution of these oligonucleotides. Here, we present whole body scanning PCR, a platform that relies on the local extraction of tissues from a mouse whole body section followed by the conversion of target-specific qPCR signals into an image. This platform was designed to be compatible with a novel RT-qPCR assay for the detection of siRNAs and with an assay suitable for the detection of heavily chemically modified oligonucleotides, which we termed Chemical-Ligation qPCR (CL-qPCR). In addition to this, the platform can also be used to investigate the global expression of endogenous mRNAs and non-coding RNAs. Incorporation of other detection systems, such as aptamers, could even further expand the use of this technology.
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Registered trials
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