ArticleArteriosclerosis, thrombosis, and vascular biology2013
2H2O-based high-density lipoprotein turnover method for the assessment of dynamic high-density lipoprotein function in mice.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- A high-fat diet increases hepatic mitochondrial turnover through restricted acetylation in a NAFLD mouse model.American journal of physiology. Endocrinology and metabolism · 2023Article
- Metabolic labeling unveils alterations in the turnover of HDL-associated proteins during diabetes progression in mice.American journal of physiology. Endocrinology and metabolism · 2022Article
- Research Progress in the Medical Application of Heavy Water, Especially in the Field of DInternational journal of medical sciences · 2022Review
- Article
- Early Pro-Inflammatory Remodeling of HDL Proteome in a Model of Diet-Induced Obesity:International journal of molecular sciences · 2020Article
- Temporal Dynamics of High-Density Lipoprotein Proteome in Diet-Controlled Subjects with Type 2 Diabetes.Biomolecules · 2020Article
- Calculation of the Protein Turnover Rate Using the Number of IncorporatedAnalytical chemistry · 2019Article
- HDL flux is higher in patients with nonalcoholic fatty liver disease.American journal of physiology. Endocrinology and metabolism · 2019Article
- From High-Density Lipoprotein Cholesterol to Measurements of Function: Prospects for the Development of Tests for High-Density Lipoprotein Functionality in Cardiovascular Disease.Arteriosclerosis, thrombosis, and vascular biology · 2018Review
- Glycation Reduces the Stability of ApoAI and Increases HDL Dysfunction in Diet-Controlled Type 2 Diabetes.The Journal of clinical endocrinology and metabolism · 2018Article
- Increased serotransferrin and ceruloplasmin turnover in diet-controlled patients with type 2 diabetes.Free radical biology & medicine · 2017Article
- Unbiased and targeted mass spectrometry for the HDL proteome.Current opinion in lipidology · 2017Review
- Monitoring the synthesis of biomolecules using mass spectrometry.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- Proteome Dynamics Reveals Pro-Inflammatory Remodeling of Plasma Proteome in a Mouse Model of NAFLD.Journal of proteome research · 2016Article
- A Western diet induced NAFLD in LDLR(-/)(-) mice is associated with reduced hepatic glutathione synthesis.Free radical biology & medicine · 2016Article
- ORMDL orosomucoid-like proteins are degraded by free-cholesterol-loading-induced autophagy.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- HDL from apoA1 transgenic mice expressing the 4WF isoform is resistant to oxidative loss of function.Journal of lipid research · 2015Article
- Ceramide as a mediator of non-alcoholic Fatty liver disease and associated atherosclerosis.PloS one · 2015Article
- Cardiac mitochondrial proteome dynamics with heavy water reveals stable rate of mitochondrial protein synthesis in heart failure despite decline in mitochondrial oxidative capacity.Journal of molecular and cellular cardiology · 2014Article
- High-density lipoprotein metabolism, composition, function, and deficiency.Current opinion in lipidology · 2014Review
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
objectiveHigh-density lipoprotein (HDL) promotes reverse cholesterol transport from peripheral tissues to the liver for clearance. Reduced HDL-cholesterol (HDLc) is associated with atherosclerosis; however, as a predictor of cardiovascular disease, HDLc has limitations because it is not a direct marker of HDL functionality. Our objective was to develop a mass spectrometry-based method for the simultaneous measurement of HDLc and ApoAI kinetics in mice, using a single (2)H2O tracer, and use it to examine genetic and drug perturbations on HDL turnover in vivo. APPROACH AND
resultsMice were given (2)H2O in the drinking water, and serial blood samples were collected at different time points. HDLc and ApoAI gradually incorporated (2)H, allowing experimental measurement of fractional catabolic rates and production rates for HDLc and ApoAI. ApoE(-/-) mice displayed increased fractional catabolic rates (P<0.01) and reduced production rates of both HDLc and ApoAI (P<0.05) compared with controls. In human ApoAI transgenic mice, levels and production rates of HDLc and human ApoAI were strikingly higher than in wild-type mice. Myriocin, an inhibitor of sphingolipid synthesis, significantly increased both HDL flux and macrophage-to-feces reverse cholesterol transport, indicating compatibility of this HDL turnover method with the macrophage-specific reverse cholesterol transport assay.
conclusions(2)H2O-labeling can be used to measure HDLc and ApoAI flux in vivo, and to assess the role of genetic and pharmacological interventions on HDL turnover in mice. Safety, simplicity, and low cost of the (2)H2O-based HDL turnover approach suggest that this assay can be scaled for human use to study effects of HDL targeted therapies on dynamic HDL function.
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