ArticleStem cells (Dayton, Ohio)2013
Defined extracellular matrix components are necessary for definitive endoderm induction.
Article in Stem cells (Dayton, Ohio), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Platr4 is an early embryonic lncRNA that exerts its function downstream on cardiogenic mesodermal lineage commitment.Developmental cell · 2022Article
- Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells.International journal of molecular sciences · 2022Article
- CCBE1 in Cardiac Development and Disease.Frontiers in genetics · 2022Review
- Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors.Frontiers in bioengineering and biotechnology · 2020Review
- A silk fibroin/decellularized extract of Wharton's jelly hydrogel intended for cartilage tissue engineering.Progress in biomaterials · 2019Article
- Cell-derived decellularized extracellular matrices.Methods in cell biology · 2018Article
- Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells.PloS one · 2018Article
- Laminin Modulates the Stem Cell Population in LM05-E Murine Breast Cancer Cells through the Activation of the MAPK/ERK Pathway.Cancer research and treatment · 2017Article
- Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells.Journal of visualized experiments : JoVE · 2017Article
- Aneuploid embryonic stem cells exhibit impaired differentiation and increased neoplastic potential.The EMBO journal · 2016Article
- Controlled microfluidics to examine growth-factor induced migration of neural progenitors in the Drosophila visual system.Journal of neuroscience methods · 2016Article
- Extracellular matrix bioscaffolds in tissue remodeling and morphogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Review
- Recombinant Laminins Drive the Differentiation and Self-Organization of hESC-Derived Hepatocytes.Stem cell reports · 2015Article
- A Closer Look at Schlemm's Canal Cell Physiology: Implications for Biomimetics.Journal of functional biomaterials · 2015Review
- Traction forces mediated by integrin signaling are necessary for definitive endoderm specification.Journal of cell science · 2015Article
- Collagen Type I Improves the Differentiation of Human Embryonic Stem Cells towards Definitive Endoderm.PloS one · 2015Article
- Unfolded protein response is required for the definitive endodermal specification of mouse embryonic stem cells via Smad2 and β-catenin signaling.The Journal of biological chemistry · 2014Article
- Stem cells in a three-dimensional scaffold environment.SpringerPlus · 2014Review
- Influence of substrate composition on human embryonic stem cell differentiation and extracellular matrix production in embryoid bodies.Biotechnology progressArticle
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Differentiation methods often rely exclusively on growth factors to direct mouse embryonic stem cell (ESC) fate, but the niche also contains fibrillar extracellular matrix (ECM) proteins, including fibronectin (FN) and laminin, which could also direct cell fate. Soluble differentiation factors are known to increase ECM expression, yet ECM's ability to direct ESC fate is not well understood. To address the extent to which these proteins regulate differentiation when assembled into a matrix, we examined mouse ESC embryoid bodies (EBs) and found that their ability to maintain pluripotency marker expression was impaired by soluble serum FN. EBs also showed a spatiotemporal correlation between expression of FN and GATA4, a marker of definitive endoderm (DE), and an inverse correlation between FN and Nanog, a pluripotency marker. Maintenance of mouse ESC pluripotency prevented fibrillar matrix production, but induction medium created lineage-specific ECM containing varying amounts of FN and laminin. Mouse ESC-derived matrix was unlike conventional fibroblast-derived matrix, which did not contain laminin. Naïve mouse ESCs plated onto ESC- and fibroblast-derived matrix exhibited composition-specific differentiation. With exogenously added laminin, fibroblast-derived matrix is more similar in composition to mouse ESC-derived matrix and lacks residual growth factors that mouse ESC matrix may contain. Naïve mouse ESCs in DE induction medium exhibited dose-dependent DE differentiation as a function of the amount of exogenous laminin in the matrix in an α3 integrin-dependent mechanism. These data imply that fibrillar FN is necessary for loss of pluripotency and that laminin within a FN matrix improves DE differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.