ArticleJournal of synchrotron radiation2013
Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline.
Article in Journal of synchrotron radiation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
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- X-ray fluorescence detection for serial macromolecular crystallography using a JUNGFRAU pixel detector.Journal of synchrotron radiation · 2020Article
- The complex analysis of X-ray mesh scans for macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2018Article
- Dynamic X-ray diffraction sampling for protein crystal positioning.Journal of synchrotron radiation · 2017Article
- Current advances in synchrotron radiation instrumentation for MX experiments.Archives of biochemistry and biophysics · 2016Review
- Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging.Journal of synchrotron radiation · 2016Article
- Fast two-dimensional grid and transmission X-ray microscopy scanning methods for visualizing and characterizing protein crystals.Journal of applied crystallography · 2016Article
- A visible-light-excited fluorescence method for imaging protein crystals without added dyes.Journal of applied crystallography · 2016Article
- Parts per Million Powder X-ray Diffraction.Analytical chemistry · 2015Article
- Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Imaging local electric fields produced upon synchrotron X-ray exposure.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Identifying, studying and making good use of macromolecular crystals.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Femtosecond crystallography of membrane proteins in the lipidic cubic phase.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Kinetic trapping of metastable amino acid polymorphs.Journal of the American Chemical Society · 2014Article
- Exploiting Microbeams for Membrane Protein Structure Determination.Advances in experimental medicine and biologyArticle
Corrections and comments
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Authors and funding
18 authors at 4 institutions in 1 country.
Funding
Abstract
Nonlinear optical (NLO) instrumentation has been integrated with synchrotron X-ray diffraction (XRD) for combined single-platform analysis, initially targeting applications for automated crystal centering. Second-harmonic-generation microscopy and two-photon-excited ultraviolet fluorescence microscopy were evaluated for crystal detection and assessed by X-ray raster scanning. Two optical designs were constructed and characterized; one positioned downstream of the sample and one integrated into the upstream optical path of the diffractometer. Both instruments enabled protein crystal identification with integration times between 80 and 150 µs per pixel, representing a ∼10(3)-10(4)-fold reduction in the per-pixel exposure time relative to X-ray raster scanning. Quantitative centering and analysis of phenylalanine hydroxylase from Chromobacterium violaceum cPAH, Trichinella spiralis deubiquitinating enzyme TsUCH37, human κ-opioid receptor complex kOR-T4L produced in lipidic cubic phase (LCP), intimin prepared in LCP, and α-cellulose samples were performed by collecting multiple NLO images. The crystalline samples were characterized by single-crystal diffraction patterns, while α-cellulose was characterized by fiber diffraction. Good agreement was observed between the sample positions identified by NLO and XRD raster measurements for all samples studied.
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