ArticleBritish journal of cancer2013
Methylation levels of LINE-1 in primary lesion and matched metastatic lesions of colorectal cancer.
Article in British journal of cancer, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 4 of them syntheses that pooled it.
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Who cites it
24 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Biomarker concordance between primary colorectal cancer and its metastases.EBioMedicine · 2019Pooled it
- The role of LINE-1 methylation in predicting survival among colorectal cancer patients: a meta-analysis.International journal of clinical oncology · 2017Pooled it
- Concordant analysis of KRAS, BRAF, PIK3CA mutations, and PTEN expression between primary colorectal cancer and matched metastases.Scientific reports · 2015Pooled it
- Pooled it
- LINE-1 methylation status and survival outcomes in colorectal cancer patients: A systematic review and meta-analysis.Heliyon · 2025Article
- Preoperative skeletal muscle status is associated with tumor-infiltrating lymphocytes and prognosis in patients with colorectal cancer.Annals of gastroenterological surgery · 2022Article
- Impact of TumorJournal of personalized medicine · 2020Article
- The transition from primary colorectal cancer to isolated peritoneal malignancy is associated with an increased tumour mutational burden.Scientific reports · 2020Article
- A Systematic Literature Review and Meta-Analysis Describing the Prevalence ofGastroenterology research · 2020Article
- LINE-1 Hypermethylation in Serum Cell-Free DNA of Relapsing Remitting Multiple Sclerosis Patients.Molecular neurobiology · 2018Article
- Article
- Impact of LINE-1 hypomethylation on the clinicopathological and molecular features of colorectal cancer patients.PloS one · 2018Article
- Decoupling of DNA methylation and activity of intergenic LINE-1 promoters in colorectal cancer.Epigenetics · 2017Article
- Translating genomic profiling to gastrointestinal cancer treatment.Future oncology (London, England) · 2017Review
- DNA Methylation and Hydroxymethylation in Primary Colon Cancer and Synchronous Hepatic Metastasis.Frontiers in genetics · 2017Article
- Technical advances in global DNA methylation analysis in human cancers.Journal of biological engineering · 2017Review
- Tumor LINE-1 Methylation Level in Association with Survival of Patients with Stage II Colon Cancer.International journal of molecular sciences · 2016Article
- Prognostic and predictive significance of long interspersed nucleotide element-1 methylation in advanced-stage colorectal cancer.BMC cancer · 2016Article
- TET family proteins and 5-hydroxymethylcytosine in esophageal squamous cell carcinoma.Oncotarget · 2015Article
- Crossing the LINE Toward Genomic Instability: LINE-1 Retrotransposition in Cancer.Frontiers in chemistry · 2015Review
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLINE-1 methylation level is a surrogate marker of global DNA methylation. LINE-1 methylation in primary colorectal cancers (CRCs) is highly variable and strongly associated with a poor prognosis. However, no study has examined LINE-1 methylation levels of metastatic CRCs in relation to prognosis or assessed the heterogeneity of LINE-1 methylation level within the primary CRCs.
methodsPyrosequencing was used to quantify LINE-1 methylation level in 42 liver metastases, 26 matched primary tumours, and 6 matched lymph node (LN) metastases. KRAS, BRAF, and PIK3CA mutation status and microsatellite instability (MSI) status were also examined.
resultsThe distribution of LINE-1 methylation level in liver metastases was as follows: mean, 67.3; range, 37.1-90.1. Primary tumours showed LINE-1 methylation levels similar to those of matched liver and LN metastases. The difference in LINE-1 methylation level between superficial areas and invasive front areas was within 7.0 in all six cases evaluated. Prognostic impact of LINE-1 hypomethylation in liver metastases on overall survival was not observed. The concordance rate was 94% for KRAS, 100% for BRAF, 88% for PIK3CA, and 97% for MSI.
conclusionAlteration of LINE-1 methylation level may occur in early CRC tumorigenesis, and the LINE-1 methylation level is relatively stable during CRC progression.
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