ArticleAmerican journal of respiratory cell and molecular biology2013
Inflammation promotes airway epithelial ATP release via calcium-dependent vesicular pathways.
Article in American journal of respiratory cell and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- Functional Tissue Analysis Reveals Successful Cryopreservation of Human Osteoarthritic Synovium.PloS one · 2016Trial
- TRPV4-driven ATP release activates CFTR through ADORA2B and P2RY2 receptors in the airway epithelium.iScience · 2026Article
- Exocytosed ATP as a therapeutic target for inflammatory and metabolic diseases.Frontiers in pharmacology · 2026Review
- Connexin-hemichannels-mediated ATP release causes lung injury following chlorine inhalation.American journal of physiology. Cell physiology · 2025Article
- Peripheral Mechanisms Underlying Bacillus Calmette-Guerin-Induced Lower Urinary Tract Symptoms (LUTS).Brain sciences · 2024Review
- Release of ATP in the lung evoked by inhalation of irritant gases in rats.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Testosterone Enhances KInternational journal of molecular sciences · 2024Article
- ATP functions as a primary alarmin in allergen-induced type 2 immunity.American journal of physiology. Cell physiology · 2023Review
- Degranulation of human mast cells: modulation by P2 receptors' agonists.Frontiers in immunology · 2023Review
- Review
- Airway mucins promote immunopathology in virus-exacerbated chronic obstructive pulmonary disease.The Journal of clinical investigation · 2022Article
- A novel definition and treatment of hyperinflammation in COVID-19 based on purinergic signalling.Purinergic signalling · 2022Review
- Identification of an ATP/P2X7/mast cell pathway mediating ozone-induced bronchial hyperresponsiveness.JCI insight · 2021Article
- Airway Epithelial Nucleotide Release Contributes to Mucociliary Clearance.Life (Basel, Switzerland) · 2021Review
- Purinergic receptors in airway hydration.Biochemical pharmacology · 2021Review
- Inhibition of ATP hydrolysis restores airway surface liquid production in cystic fibrosis airway epithelia.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- A microphysiological model of the bronchial airways reveals the interplay of mechanical and biochemical signals in bronchospasm.Nature biomedical engineering · 2019Article
- Inflammation-induced upregulation of P2XAmerican journal of physiology. Lung cellular and molecular physiology · 2019Article
- Expression and function of Anoctamin 1/TMEM16A calcium-activated chloride channels in airways of in vivo mouse models for cystic fibrosis research.Pflugers Archiv : European journal of physiology · 2018Article
- Stimulation of TLR3 triggers release of lysosomal ATP in astrocytes and epithelial cells that requires TRPML1 channels.Scientific reports · 2018Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
ATP in airway surface liquid (ASL) controls mucociliary clearance functions via the activation of airway epithelial purinergic receptors. However, abnormally elevated ATP levels have been reported in inflamed airways, suggesting that excessive ATP in ASL contributes to airway inflammation. Despite these observations, little is known about the mechanisms of ATP accumulation in the ASL covering inflamed airways. In this study, links between cystic fibrosis (CF)-associated airway inflammation and airway epithelial ATP release were investigated. Primary human bronchial epithelial (HBE) cells isolated from CF lungs exhibited enhanced IL-8 secretion after 6 to 11 days, but not 28 to 35 days, in culture, compared with normal HBE cells. Hypotonic cell swelling-promoted ATP release was increased in 6- to 11-day-old CF HBE cells compared with non-CF HBE cells, but returned to normal values after 28 to 35 days in culture. The exposure of non-CF HBE cells to airway secretions isolated from CF lungs, namely, sterile supernatants of mucopurulent material (SMM), also caused enhanced IL-8 secretion and increased ATP release. The SMM-induced increase in ATP release was sensitive to Ca(2+) chelation and vesicle trafficking/exocytosis inhibitors, but not to pannexin inhibition. Transcript levels of the vesicular nucleotide transporter, but not pannexin 1, were up-regulated after SMM exposure. SMM-treated cultures displayed increased basal mucin secretion, but mucin secretion was not enhanced in response to hypotonic challenge after the exposure of cells to either vehicle or SMM. We propose that CF airway inflammation up-regulates the capacity of airway epithelia to release ATP via Ca(2+)-dependent vesicular mechanisms not associated with mucin granule secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.