ArticleBioMed research international2013
Production, purification, and characterization of a major Penicillium glabrum xylanase using Brewer's spent grain as substrate.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 81 citations in OpenAlex.
- Clustered surface amino acid residues modulate the acid stability of GH10 xylanase in fungi.Applied microbiology and biotechnology · 2024Article
- A Glycosyl Hydrolase 30 Family Xylanase from the Rumen Metagenome and Its Effects on In Vitro Ruminal Fermentation of Wheat Straw.Animals : an open access journal from MDPI · 2023Article
- Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean HullsFood technology and biotechnology · 2023Article
- Article
- Cultivable Fungal Endophytes in Roots, Rhizomes and Leaves ofPlants (Basel, Switzerland) · 2022Article
- Use of Wheat Straw for Value-Added Product Xylanase byJournal of fungi (Basel, Switzerland) · 2021Article
- Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum.Current microbiology · 2021Article
- Recent advances in biotechnological valorization of brewers' spent grain.Food science and biotechnology · 2021Review
- Cloning, expression and characterization of C. crescentus xynA2 gene and application of Xylanase II in the deconstruction of plant biomass.Molecular biology reports · 2020Article
- A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes.Bioengineering (Basel, Switzerland) · 2018Review
- Molecular structure and catalytic mechanism of fungal family G acidophilic xylanases.3 Biotech · 2018Review
- Purification, characterization, gene cloning and expression of GH-10 xylanase (Penicillium citrinum isolate HZN13).3 Biotech · 2016Article
- Bioprocessing of wheat bran for the production of lignocellulolytic enzyme cocktail by Cotylidia pannosa under submerged conditions.Bioengineered · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase from P. glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn(2+) and the reducing agents β -mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg(2+), Zn(2+), and Cu(2+) as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost.
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