ArticlePloS one2013
Real time measures of prestin charge and fluorescence during plasma membrane trafficking reveal sub-tetrameric activity.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Single particle cryo-EM structure of the outer hair cell motor protein prestin.Nature communications · 2022Article
- Prestin amplifies cardiac motor functions.Cell reports · 2021Article
- Maturation of Voltage-induced Shifts in SLC26a5 (Prestin) Operating Point during Trafficking and Membrane Insertion.Neuroscience · 2020Article
- Current carried by the Slc26 family member prestin does not flow through the transporter pathway.Scientific reports · 2017Article
- Membrane prestin expression correlates with the magnitude of prestin-associated charge movement.Hearing research · 2016Article
- Extracellular Cl(-) regulates human SO4 (2-)/anion exchanger SLC26A1 by altering pH sensitivity of anion transport.Pflugers Archiv : European journal of physiology · 2016Article
- Tyrosine motifs are required for prestin basolateral membrane targeting.Biology open · 2015Article
- Prestin at year 14: progress and prospect.Hearing research · 2014Review
- Acoustic input and efferent activity regulate the expression of molecules involved in cochlear micromechanics.Frontiers in systems neuroscience · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Prestin (SLC26a5) is the outer hair cell integral membrane motor protein that drives cochlear amplification, and has been described as an obligate tetramer. We studied in real time the delivery of YFP-prestin to the plasma membrane of cells from a tetracycline-inducible cell line. Following the release of temperature block to reinstate trans Golgi network delivery of the integral membrane protein, we measured nonlinear capacitance (NLC) and membrane fluorescence during voltage clamp. Prestin was delivered exponentially to the plasma membrane with a time constant of less than 10 minutes, with both electrical and fluorescence methods showing high temporal correlation. However, based on disparity between estimates of prestin density derived from either fluorescence or NLC, we conclude that sub-tetrameric forms of prestin contribute to our electrical and fluorescence measures. Thus, in agreement with previous observations we find that functional prestin is not an obligate tetramer.
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Registered trials
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