ArticlePloS one2013
Tricarboxylic acid cycle and one-carbon metabolism pathways are important in Edwardsiella ictaluri virulence.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Development of Bioluminescent VirulentPathogens (Basel, Switzerland) · 2023Article
- Hemolysin Co-regulated Family Proteins Hcp1 and Hcp2 Contribute toFrontiers in veterinary science · 2021Article
- Functional Identification of Serine Hydroxymethyltransferase as a Key Gene Involved in Lysostaphin Resistance and Virulence Potential ofInternational journal of molecular sciences · 2020Article
- Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri.BMC microbiology · 2019Article
- Construction and evaluation of type III secretion system mutants of the catfish pathogen Edwardsiella piscicida.Journal of fish diseases · 2018Article
- Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori.PloS one · 2018Article
- Article
- Isocitrate dehydrogenase mutation in Vibrio anguillarum results in virulence attenuation and immunoprotection in rainbow trout (Oncorhynchus mykiss).BMC microbiology · 2017Article
- An integrated computational-experimental approach reveals Yersinia pestis genes essential across a narrow or a broad range of environmental conditions.BMC microbiology · 2017Article
- Phagocytic and Bactericidal Properties of Channel Catfish Peritoneal Macrophages Exposed toFrontiers in microbiology · 2017Article
- Comparative Analysis of theFrontiers in microbiology · 2017Article
- Article
- The TCA Pathway is an Important Player in the Regulatory Network Governing Vibrio alginolyticus Adhesion Under Adversity.Frontiers in microbiology · 2016Article
- Commensal and Pathogenic Escherichia coli Metabolism in the Gut.Microbiology spectrum · 2015Article
- The contribution of the glycine cleavage system to the pathogenesis of Francisella tularensis.Microbes and infection · 2014Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Edwardsiella ictaluri is a Gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (ESC). The disease causes considerable economic losses in the commercial catfish industry in the United States. Although antibiotics are used as feed additive, vaccination is a better alternative for prevention of the disease. Here we report the development and characterization of novel live attenuated E. ictaluri mutants. To accomplish this, several tricarboxylic acid cycle (sdhC, mdh, and frdA) and one-carbon metabolism genes (gcvP and glyA) were deleted in wild type E. ictaluri strain 93-146 by allelic exchange. Following bioluminescence tagging of the E. ictaluri ΔsdhC, Δmdh, ΔfrdA, ΔgcvP, and ΔglyA mutants, their dissemination, attenuation, and vaccine efficacy were determined in catfish fingerlings by in vivo imaging technology. Immunogenicity of each mutant was also determined in catfish fingerlings. Results indicated that all of the E. ictaluri mutants were attenuated significantly in catfish compared to the parent strain as evidenced by 2,265-fold average reduction in bioluminescence signal from all the mutants at 144 h post-infection. Catfish immunized with the E. ictaluri ΔsdhC, Δmdh, ΔfrdA, and ΔglyA mutants had 100% relative percent survival (RPS), while E. ictaluri ΔgcvP vaccinated catfish had 31.23% RPS after re-challenge with the wild type E. ictaluri.
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