ArticlePloS one2013
A parameterized model of amylopectin synthesis provides key insights into the synthesis of granular starch.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 170 citations in OpenAlex.
- In Planta Expression of Thermophilic GtGBE Mimics BEIIb and Modifies Rice Starch Structure Without Yield Penalties.Plant biotechnology journal · 2026Article
- Investigating the Impact of Amylopectin Chain-Length Distribution on the Structural and Functional Properties of Waxy Rice Starch.Foods (Basel, Switzerland) · 2025Article
- A Starch Molecular Explanation for Effects of Ageing Temperature on Pasting Property, Digestibility, and Texture of Rice Grains.Foods (Basel, Switzerland) · 2025Article
- Accounting for shear scission in the size-exclusion chromatography of debranched starch.Analytical and bioanalytical chemistry · 2025Article
- Grain under pressure: Harnessing biochemical pathways to beat drought and heat in wheat.The Plant journal : for cell and molecular biology · 2025Review
- Dynamics of starch formation and gene expression during grain filling and its possible influence on grain quality.Scientific reports · 2024Article
- Effects of the Molecular Structure of Starch in Foods on Human Health.Foods (Basel, Switzerland) · 2023Review
- The Effects of Starch Molecular Fine Structure on Thermal and Digestion Properties of Rice Starch.Foods (Basel, Switzerland) · 2022Review
- Starch Molecular Structural Features and Volatile Compounds Affecting the Sensory Properties of Polished Australian Wild Rice.Foods (Basel, Switzerland) · 2022Article
- Testing the Linearity Assumption for Starch Structure-Property Relationships in Rices.Frontiers in nutrition · 2022Article
- Relationship between starch fine structure and simulated oral processing of cookedFrontiers in nutrition · 2022Article
- Effects of Nonstarch Genetic Modifications on Starch Structure and Properties.Foods (Basel, Switzerland) · 2020Article
- Competition between Granule Bound Starch Synthase and Starch Branching Enzyme in Starch Biosynthesis.Rice (New York, N.Y.) · 2019Article
- Ultra-high performance liquid chromatography-size exclusion chromatography (UPLC-SEC) as an efficient tool for the rapid and highly informative characterisation of biopolymers.Carbohydrate polymers · 2018Article
- On the Role of Catabolic Enzymes in Biosynthetic Models of Glycogen Molecular Weight Distributions.ACS omega · 2017Article
- Article
- Expression of an (Engineered) 4,6-α-Glucanotransferase in Potato Results in Changes in Starch Characteristics.PloS one · 2016Article
- Review
- Improved understanding of rice amylose biosynthesis from advanced starch structural characterization.Rice (New York, N.Y.) · 2015Article
- The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions.PloS one · 2015Article
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A core set of genes involved in starch synthesis has been defined by genetic studies, but the complexity of starch biosynthesis has frustrated attempts to elucidate the precise functional roles of the enzymes encoded. The chain-length distribution (CLD) of amylopectin in cereal endosperm is modeled here on the basis that the CLD is produced by concerted actions of three enzyme types: starch synthases, branching and debranching enzymes, including their respective isoforms. The model, together with fitting to experiment, provides four key insights. (1) To generate crystalline starch, defined restrictions on particular ratios of enzymatic activities apply. (2) An independent confirmation of the conclusion, previously reached solely from genetic studies, of the absolute requirement for debranching enzyme in crystalline amylopectin synthesis. (3) The model provides a mechanistic basis for understanding how successive arrays of crystalline lamellae are formed, based on the identification of two independent types of long amylopectin chains, one type remaining in the amorphous lamella, while the other propagates into, and is integral to the formation of, an adjacent crystalline lamella. (4) The model provides a means by which a small number of key parameters defining the core enzymatic activities can be derived from the amylopectin CLD, providing the basis for focusing studies on the enzymatic requirements for generating starches of a particular structure. The modeling approach provides both a new tool to accelerate efforts to understand granular starch biosynthesis and a basis for focusing efforts to manipulate starch structure and functionality using a series of testable predictions based on a robust mechanistic framework.
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