ArticlePloS one2013
Distinct redox regulation in sub-cellular compartments in response to various stress conditions in Saccharomyces cerevisiae.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Systematic engineering to enhance 3-carene production in yeast.Engineering microbiology · 2026Article
- Live Visualization of Endoplasmic Reticulum Redox Potential in Zebrafish Embryos Reveals Region-Specific Heterogeneity.Biomedicines · 2026Article
- Dynamic assembly of malate dehydrogenase-citrate synthase multienzyme complex in the mitochondria.eLife · 2026Article
- An improved fluorescent indicator for monitoring intracellular redox potential.Redox biology · 2026Article
- TOR and heat shock response pathways regulate peroxisome biogenesis during proteotoxic stress.Nature communications · 2025Article
- Disorders of Redox Homeostasis and Its Importance in Acrolein Toxicity.International journal of molecular sciences · 2025Article
- Review
- Strategies to Maintain Redox Homeostasis in Yeast Cells with Impaired Fermentation-Dependent NADPH Generation.International journal of molecular sciences · 2024Article
- Progress in pH-Sensitive sensors: essential tools for organelle pH detection, spotlighting mitochondrion and diverse applications.Frontiers in pharmacology · 2023Review
- Organelle Engineering in Yeast: Enhanced Production of Protopanaxadiol through Manipulation of Peroxisome Proliferation inMicroorganisms · 2022Article
- Protective Effects of Melatonin onAntioxidants (Basel, Switzerland) · 2021Article
- Advances in engineering microbial biosynthesis of aromatic compounds and related compounds.Bioresources and bioprocessing · 2021Review
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Dynamic imaging of cellular pH and redox homeostasis with a genetically encoded dual-functional biosensor, pHaROS, in yeast.The Journal of biological chemistry · 2019Article
- Harnessing yeast organelles for metabolic engineering.Nature chemical biology · 2017Review
- During yeast chronological aging resveratrol supplementation results in a short-lived phenotype Sir2-dependent.Redox biology · 2017Article
- Live-cell imaging approaches for the investigation of xenobiotic-induced oxidant stress.Biochimica et biophysica acta · 2016Review
- Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways.Nature communications · 2016Article
- Retro-translocation of mitochondrial intermembrane space proteins.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Introduction to Oxidative Stress in Biomedical and Biological Research.Biomolecules · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Responses to many growth and stress conditions are assumed to act via changes to the cellular redox status. However, direct measurement of pH-adjusted redox state during growth and stress has never been carried out. Organellar redox state (E GSH) was measured using the fluorescent probes roGFP2 and pHluorin in Saccharomyces cerevisiae. In particular, we investigated changes in organellar redox state in response to various growth and stress conditions to better understand the relationship between redox-, oxidative- and environmental stress response systems. E GSH values of the cytosol, mitochondrial matrix and peroxisome were determined in exponential and stationary phase in various media. These values (-340 to -350 mV) were more reducing than previously reported. Interestingly, sub-cellular redox state remained unchanged when cells were challenged with stresses previously reported to affect redox homeostasis. Only hydrogen peroxide and heat stress significantly altered organellar redox state. Hydrogen peroxide stress altered the redox state of the glutathione disulfide/glutathione couple (GSSG, 2H(+)/2GSH) and pH. Recovery from moderate hydrogen peroxide stress was most rapid in the cytosol, followed by the mitochondrial matrix, with the peroxisome the least able to recover. Conversely, the bulk of the redox shift observed during heat stress resulted from alterations in pH and not the GSSG, 2H(+)/2GSH couple. This study presents the first direct measurement of pH-adjusted redox state in sub-cellular compartments during growth and stress conditions. Redox state is distinctly regulated in organelles and data presented challenge the notion that perturbation of redox state is central in the response to many stress conditions.
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