ArticleFrontiers in plant science2013
Using μPIXE for quantitative mapping of metal concentration in Arabidopsis thaliana seeds.
Article in Frontiers in plant science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Pixel-registered multimodal synchrotron XRF and FTIR microscopies reveal salinity stress response mechanisms in pistachio.Plant methods · 2026Article
- Multimodal synchrotron X-ray fluorescence imaging reveals elemental distribution in seeds and seedlings of the Zn-Cd-Ni hyperaccumulator Noccaea caerulescens.Metallomics : integrated biometal science · 2022Article
- The Conservation of VIT1-Dependent Iron Distribution in Seeds.Frontiers in plant science · 2019Article
- Characterization of Arsenic in dried baby shrimp (Journal of analytical atomic spectrometry · 2018Article
- Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds.Plant physiology · 2018Article
- The stage of seed development influences iron bioavailability in pea (Pisum sativum L.).Scientific reports · 2018Article
- Laboratory Microprobe X-Ray Fluorescence in Plant Science: Emerging Applications and Case Studies.Frontiers in plant science · 2018Article
- The Diverse Iron Distribution in Eudicotyledoneae Seeds: From Arabidopsis to Quinoa.Frontiers in plant science · 2018Article
- The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds.Scientific reports · 2017Article
- Metal Tolerance Protein 8 Mediates Manganese Homeostasis and Iron Reallocation during Seed Development and Germination.Plant physiology · 2017Article
- Analytical Methods for Imaging Metals in Biology: From Transition Metal Metabolism to Transition Metal Signaling.Analytical chemistry · 2017Review
- Dynamic Subcellular Localization of Iron during Embryo Development in Brassicaceae Seeds.Frontiers in plant science · 2017Article
- Bypassing Iron Storage in Endodermal Vacuoles Rescues the Iron Mobilization Defect in the natural resistance associated-macrophage protein3natural resistance associated-macrophage protein4 Double Mutant.Plant physiology · 2015Article
- Iron in seeds - loading pathways and subcellular localization.Frontiers in plant science · 2014Review
- Mn-euvering manganese: the role of transporter gene family members in manganese uptake and mobilization in plants.Frontiers in plant science · 2014Review
- Cellular iron homeostasis and metabolism in plant.Frontiers in plant science · 2013Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Seeds are a crucial stage in plant life. They contain the nutrients necessary to initiate the development of a new organism. Seeds also represent an important source of nutrient for human beings. Iron (Fe) and zinc (Zn) deficiencies affect over a billion people worldwide. It is therefore important to understand how these essential metals are stored in seeds. In this work, Particle-Induced X-ray Emission with the use of a focused ion beam (μPIXE) has been used to map and quantify essential metals in Arabidopsis seeds. In agreement with Synchrotron radiation X-ray fluorescence (SXRF) imaging and Perls/DAB staining, μPIXE maps confirmed the specific pattern of Fe and Mn localization in the endodermal and subepidermal cell layers in dry seeds, respectively. Moreover, μPIXE allows absolute quantification revealing that the Fe concentration in the endodermal cell layer reaches ~800 μg·g(-1) dry weight. Nevertheless, this cell layer accounts only for about half of Fe stores in dry seeds. Comparison between Arabidopsis wild type (WT) and mutant seeds impaired in Fe vacuolar storage (vit1-1) or release (nramp3nramp4) confirmed the strongly altered Fe localization pattern in vit1-1, whereas no alteration could be detected in nramp3nramp4 dry seeds. Imaging of imbibed seeds indicates a dynamic localization of metals as Fe and Zn concentrations increase in the subepidermal cell layer of cotyledons after imbibition. The complementarities between μPIXE and other approaches as well as the importance of being able to quantify the patterns for the interpretation of mutant phenotypes are discussed.
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