ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2013
Short-term bed rest increases TLR4 and IL-6 expression in skeletal muscle of older adults.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00690534 (Insulin and Sarcopenia in the Elderly), which is not on this map. Cited by 55 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Insulin and Sarcopenia in the Elderly
Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 95 citations in OpenAlex.
- The Aging Muscle in Experimental Bed Rest: A Systematic Review and Meta-Analysis.Frontiers in nutrition · 2021Pooled it
- Metabolic Implications of Diet and Energy Intake during Physical Inactivity.Medicine and science in sports and exercise · 2019Trial
- Skeletal muscle ceramides and relationship with insulin sensitivity after 2 weeks of simulated sedentary behaviour and recovery in healthy older adults.The Journal of physiology · 2018Trial
- Neuromuscular Electrical Stimulation Combined with Protein Ingestion Preserves Thigh Muscle Mass But Not Muscle Function in Healthy Older Adults During 5 Days of Bed Rest.Rejuvenation research · 2017Trial
- Impact of 14 days of head-down bed rest and an exercise countermeasure on skeletal muscle atrophy, proteome and circulatory cytokines in older adults.Experimental physiology · 2026Article
- The effects of bed rest on cardiometabolic health: A systematic review and meta-analysis.Experimental physiology · 2026Article
- Daily blood flow restriction does not preserve muscle mass and strength during 2 weeks of bed rest.The Journal of physiology · 2025Article
- Unilateral hindlimb ischaemia-induced systemic inflammation is associated with non-ischaemic skeletal muscle inflammation.Experimental physiology · 2024Article
- Extensive dynamic changes in the human transcriptome and its circadian organization during prolonged bed rest.iScience · 2024Article
- Roles of Myokines and Muscle-Derived Extracellular Vesicles in Musculoskeletal Deterioration under Disuse Conditions.Metabolites · 2024Review
- Elevated biomarkers of neural injury in older adults following head-down bed rest: links to cardio-postural deconditioning with spaceflight and aging.Frontiers in human neuroscience · 2023Article
- Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ.Inflammation · 2022Article
- Decoding accelerometry for classification and prediction of critically ill patients with severe brain injury.Scientific reports · 2021Article
- Reduced Physical Activity Alters the Leucine-Stimulated Translatome in Aged Skeletal Muscle.The journals of gerontology. Series A, Biological sciences and medical sciences · 2021Article
- Early Deconditioning of Human Skeletal Muscles and the Effects of a Thigh Cuff Countermeasure.International journal of molecular sciences · 2021Article
- Importance of hyperglycemia in COVID-19 intensive-care patients: Mechanism and treatment strategy.Primary care diabetes · 2021Review
- Short-term metformin ingestion by healthy older adults improves myoblast function.American journal of physiology. Cell physiology · 2021Article
- Effects of Exercise Interventions on Inflammatory Parameters in Acutely Hospitalized Older Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.Journal of clinical medicine · 2021Review
- Obesity as an additional factor for autonomic imbalance and poor sleep behavior in chronic obstructive pulmonary disease: a case-control study.Clinics (Sao Paulo, Brazil) · 2021Article
- Effects of aging and lifelong aerobic exercise on expression of innate immune components in human skeletal muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Bed rest induces significant loss of leg lean mass in older adults. Systemic and tissue inflammation also accelerates skeletal muscle loss, but it is unknown whether inflammation is associated to inactivity-induced muscle atrophy in healthy older adults. We determined if short-term bed rest increases toll-like receptor 4 (TLR4) signaling and pro-inflammatory markers in older adult skeletal muscle biopsy samples. Six healthy, older adults underwent seven consecutive days of bed rest. Muscle biopsies (vastus lateralis) were taken after an overnight fast before and at the end of bed rest. Serum cytokine expression was measured before and during bed rest. TLR4 signaling and cytokine mRNAs associated with pro- and anti-inflammation and anabolism were measured in muscle biopsy samples using Western blot analysis and qPCR. Participants lost ∼4% leg lean mass with bed rest. We found that after bed rest, muscle levels of TLR4 protein expression and interleukin-6 (IL-6), nuclear factor-κB1, interleukin-10, and 15 mRNA expression were increased after bed rest (P < 0.05). Additionally, the cytokines interferon-γ, and macrophage inflammatory protein-1β, were elevated in serum samples following bed rest (P < 0.05). We conclude that short-term bed rest in older adults modestly increased some pro- and anti-inflammatory cytokines in muscle samples while systemic changes in pro-inflammatory cytokines were mostly absent. Upregulation of TLR4 protein content suggests that bed rest in older adults increases the capacity to mount an exaggerated, and perhaps unnecessary, inflammatory response in the presence of specific TLR4 ligands, e.g., during acute illness.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.