ArticleNucleic acids research2013
Initiating nucleotide identity determines efficiency of RNA synthesis from 6S RNA templates in Bacillus subtilis but not Escherichia coli.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- 6S-1 pRNA 9-mers are a prominent length species during outgrowth ofRNA biology · 2025Article
- Processing and decay of 6S-1 and 6S-2 RNAs inRNA (New York, N.Y.) · 2023Article
- Article
- Involvement ofInternational journal of molecular sciences · 2022Article
- Structural and Functional Insight into the Mechanism ofNon-coding RNA · 2022Article
- Diverse molecular mechanisms of transcription regulation by the bacterial alarmone ppGpp.Molecular microbiology · 2022Review
- 6S-2 RNA deletion in the undomesticatedRNA biology · 2021Article
- Downstream sequence-dependent RNA cleavage and pausing by RNA polymerase I.The Journal of biological chemistry · 2020Article
- Small RNAs Involved in Regulation of Nitrogen Metabolism.Microbiology spectrum · 2018Review
- 6S RNA, a Global Regulator of Transcription.Microbiology spectrum · 2018Review
- 6S RNA plays a role in recovery from nitrogen depletion in Synechocystis sp. PCC 6803.BMC microbiology · 2017Article
- 6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype.RNA biology · 2017Article
- Bacillus subtilis 6S-2 RNA serves as a template for short transcripts in vivo.RNA (New York, N.Y.) · 2016Article
- In vitro characterization of 6S RNA release-defective mutants uncovers features of pRNA-dependent release from RNA polymerase in E. coli.RNA (New York, N.Y.) · 2014Article
- Global regulation of transcription by a small RNA: a quantitative view.Biophysical journal · 2014Article
- Mechanistic comparison of Bacillus subtilis 6S-1 and 6S-2 RNAs--commonalities and differences.RNA (New York, N.Y.) · 2014Article
- Regulation of transcription by 6S RNAs: insights from the Escherichia coli and Bacillus subtilis model systems.RNA biology · 2014Review
- Sibling rivalry: related bacterial small RNAs and their redundant and non-redundant roles.Frontiers in cellular and infection microbiology · 2014Review
- Dissemination of 6S RNA among bacteria.RNA biology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The 6S RNA is a non-coding small RNA that binds within the active site of housekeeping forms of RNA polymerases (e.g. Eσ(70) in Escherichia coli, Eσ(A) in Bacillus subtilis) and regulates transcription. Efficient release of RNA polymerase from 6S RNA regulation during outgrowth from stationary phase is dependent on use of 6S RNA as a template to generate a product RNA (pRNA). Interestingly, B. subtilis has two 6S RNAs, 6S-1 and 6S-2, but only 6S-1 RNA appears to be used efficiently as a template for pRNA synthesis during outgrowth. Here, we demonstrate that the identity of the initiating nucleotide is particularly important for the B. subtilis RNA polymerase to use RNA templates. Specifically, initiation with guanosine triphosphate (GTP) is required for efficient pRNA synthesis, providing mechanistic insight into why 6S-2 RNA does not support robust pRNA synthesis as it initiates with adenosine triphosphate (ATP). Intriguingly, E. coli RNA polymerase does not have a strong preference for initiating nucleotide identity. These observations highlight an important difference in biochemical properties of B. subtilis and E. coli RNA polymerases, specifically in their ability to use RNA templates efficiently, which also may reflect the differences in GTP and ATP metabolism in these two organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.