ArticleJournal of clinical microbiology2013
Multiplex real-time PCR for detection of Campylobacter, Salmonella, and Shigella.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Detection and Characterization of Salmonella enterica Serotypes by Simple PCR Technologies.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Singleplex real-time PCR and duplex PCR platform for the rapid detection of hypervirulentFrontiers in veterinary science · 2025Article
- Article
- A novel approach for simultaneous detection of the most common food-borne pathogens by multiplex qPCR.Biomolecules & biomedicine · 2023Article
- Prevalence and risk factors associated with the occurrence ofFrontiers in public health · 2023Article
- A Cutoff Determination of Real-Time Loop-Mediated Isothermal Amplification (LAMP) for End-Point Detection ofVeterinary sciences · 2022Article
- Development of a Duplex TaqMan Real-Time Polymerase Chain Reaction for Accurate Identification and Quantification ofFoods (Basel, Switzerland) · 2022Article
- Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification.Journal of fluorescence · 2022Article
- Article
- Characterization of Extended-Spectrum Cephalosporin (ESC) Resistance inAnimals : an open access journal from MDPI · 2021Article
- Transcriptomic Analysis, Motility and Biofilm Formation Characteristics ofInternational journal of molecular sciences · 2020Article
- Molecular Detection and Epidemiological Features of Selected Bacterial, Viral, and Parasitic Enteropathogens in Stool Specimens from Children with Acute Diarrhea in Thi-Qar Governorate, Iraq.International journal of environmental research and public health · 2019Article
- Effects of Different Papua New Guinea Sweetpotato Varieties on Performance and Level of Enteric Pathogens in Chickens.Animals : an open access journal from MDPI · 2019Article
- Prevalence, antimicrobial resistance, and virulence-associated genes ofIranian journal of veterinary research · 2019Article
- The other Campylobacters: Not innocent bystanders in endemic diarrhea and dysentery in children in low-income settings.PLoS neglected tropical diseases · 2018Article
- Developments in Rapid Detection Methods for the Detection of FoodborneFrontiers in microbiology · 2018Review
- Advanced biosensors for detection of pathogens related to livestock and poultry.Veterinary research · 2017Review
- Molecular Differentiation ofEuropean journal of microbiology & immunology · 2016Article
- Evaluation of a multiplex PCR assay for detection of cytomegalovirus in stool samples from patients with ulcerative colitis.World journal of gastroenterology · 2015Article
- Association Between Shigella Infection and Diarrhea Varies Based on Location and Age of Children.The American journal of tropical medicine and hygiene · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Infectious diarrhea can be classified based on its clinical presentation as noninflammatory or inflammatory disease. In developing countries, among inflammatory diarrhea cases, Shigella is the most common cause, followed by Campylobacter and Salmonella. Because the time frame in which treatment choices must be made is short and conventional stool cultures lack good sensitivity, there is a need for a rapid, sensitive, and inexpensive detection technique. The purpose of our study was to develop a multiplex real-time PCR procedure to simultaneously identify Campylobacter spp., Salmonella spp., and Shigella spp. Primers were designed to amplify the invA, ipaH, and 16S rRNA genes simultaneously in a single reaction to detect Salmonella, Shigella, and Campylobacter, respectively. Using this approach, we correctly identified 102 of 103 strains of the targeted enteropathogens and 34 of 34 other pathogens. The melting temperatures were 82.96 ± 0.05 °C for invA, 85.56 ± 0.28 °C for ipaH, and 89.21 ± 0.24 °C for 16S rRNA. The limit of accurate quantification for the assay in stool samples was 10(4) CFU g(-1); however, the limit of detection was 10(3) CFU g(-1). This assay is a simple, rapid, inexpensive, and reliable system for the practical detection of these three enteropathogens in clinical specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.