ArticleMolecular biology of the cell2013
A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed.
- Technologies to measure and modulate protein subcellular localization.Nature reviews. Molecular cell biology · 2026Review
- Optogenetic engineering of synthetic and natural receptors: design principles, functional mechanisms and biomedical applications.Regenerative biomaterials · 2026Review
- Characterizing Optogenetic Tools for Use in Synthetic Gene Circuits.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth.Nature communications · 2025Article
- Lighting up yeast: overview of optogenetics in yeast and their applications to yeast biotechnology.FEMS yeast research · 2025Review
- The Yeast Optogenetic Toolkit (yOTK) for Spatiotemporal Control of Gene Expression in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Live cell microscopy: From image to insight.Biophysics reviews · 2022Review
- Microridge-like structures anchor motile cilia.Nature communications · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners.aBIOTECH · 2021Article
- Article
- Article
- Unifying the mechanism of mitotic exit control in a spatiotemporal logical model.PLoS biology · 2020Article
- Near-Infrared Markers based on Bacterial Phytochromes with Phycocyanobilin as a Chromophore.International journal of molecular sciences · 2019Article
- Photodimerization systems for regulating protein-protein interactions with light.Current opinion in structural biology · 2019Review
- Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution.ACS synthetic biology · 2018Article
- Article
- Light-activated protein interaction with high spatial subcellular confinement.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Programming Bacteria With Light-Sensors and Applications in Synthetic Biology.Frontiers in microbiology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Protein localization plays a central role in cell biology. Although powerful tools exist to assay the spatial and temporal dynamics of proteins in living cells, our ability to control these dynamics has been much more limited. We previously used the phytochrome B- phytochrome-interacting factor light-gated dimerization system to recruit proteins to the plasma membrane, enabling us to control the activation of intracellular signals in mammalian cells. Here we extend this approach to achieve rapid, reversible, and titratable control of protein localization for eight different organelles/positions in budding yeast. By tagging genes at the endogenous locus, we can recruit proteins to or away from their normal sites of action. This system provides a general strategy for dynamically activating or inactivating proteins of interest by controlling their localization and therefore their availability to binding partners and substrates, as we demonstrate for galactose signaling. More importantly, the temporal and spatial precision of the system make it possible to identify when and where a given protein's activity is necessary for function, as we demonstrate for the mitotic cyclin Clb2 in nuclear fission and spindle stabilization. Our light-inducible organelle-targeting system represents a powerful approach for achieving a better understanding of complex biological systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.