ArticleScientific reports2013
Production of biallelic CMP-Neu5Ac hydroxylase knock-out pigs.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 95 citations in OpenAlex.
- A bibliometric mapping of advancements and trends in genome editing in pigs.Tropical animal health and production · 2025Review
- Current Techniques of Gene Editing in Pigs for Xenotransplantation.Transplant international : official journal of the European Society for Organ Transplantation · 2025Review
- Immunoprotection Strategies in β-Cell Replacement Therapy: A Closer Look at Porcine Islet Xenotransplantation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- [Xenotransplantation of solid organs].Chirurgie (Heidelberg, Germany) · 2024Review
- How should cardiac xenotransplantation be initiated in Japan?Surgery today · 2024Review
- Cardiac Xenotransplantation: A Narrative Review.Reviews in cardiovascular medicine · 2024Review
- Current Status of Cardiac Xenotransplantation: Report of a Workshop of the German Heart Transplant Centers, Martinsried, March 3, 2023.The Thoracic and cardiovascular surgeon · 2024Article
- Cardiac xenotransplantation: from concept to clinic.Cardiovascular research · 2023Review
- A desirable transgenic strategy using GGTA1 endogenous promoter-mediated knock-in for xenotransplantation model.Scientific reports · 2022Article
- Advances in pig models of human diseases.Animal models and experimental medicine · 2022Review
- Use of Genome Editing Techniques to Produce Transgenic Farm Animals.Advances in experimental medicine and biology · 2022Review
- Histotripsy Ablation in Preclinical Animal Models of Cancer and Spontaneous Tumors in Veterinary Patients: A Review.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2022Review
- A Water Soluble PdChemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Human immune reactivity of GGTA1/CMAH/A3GALT2 triple knockout Yucatan miniature pigs.Transgenic research · 2021Article
- Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset.ILAR journal · 2020Review
- Review
- Genetically modified pigs as donors of cells, tissues, and organs for xenotransplantation.Animal frontiers : the review magazine of animal agriculture · 2019Article
- Intestine specific regulation of pig cytidine-5'-monophospho-N-acetylneuraminic acid hydroxylase gene for N-glycolylneuraminic acid biosynthesis.Scientific reports · 2019Article
- Xenotransplantation: Current Status in Preclinical Research.Frontiers in immunology · 2019Review
- Application of genome-editing systems to enhance available pig resources for agriculture and biomedicine.Reproduction, fertility, and development · 2019Review
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After the knock-out (KO) of α1,3 galactosyltransfease (Gal-T), the Hanganutziu-Deicher antigen became a major antigen of the "non-Gal antigen" that is implicated in subsequent xenograft rejection. For deletion of non-Gal antigen, we successfully produced zinc finger nuclease (ZFN)-mediated monoallelic/biallelic male and female CMP-N-acetylneuraminic acid hydroxylase (CMAH) KO miniature pigs: the efficiency of the gene targeting (41.7%) was higher when donor DNA was used with the ZFN than those of ZFN alone (9.1%). Monoallelic KO pigs had no integration of exogenous DNA into their genome, indicating that this technique would provide a new avenue to reduce the risk of antibiotics resistance when organs from genetically modified pigs are transplanted into patients. Until now, both monoallelic and biallelic CMAH KO pigs are healthy and show no sign of abnormality and off-target mutations. Therefore, these CMAH null pigs on the Gal-T KO background could serve as an important model for the xenotransplantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.