ArticleStem cell research & therapy2013
Conditionally immortalized stem cell lines from human spinal cord retain regional identity and generate functional V2a interneurons and motorneurons.
Article in Stem cell research & therapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Chx10+V2a interneurons in spinal motor regulation and spinal cord injury.Neural regeneration research · 2023Review
- Immortalization Reversibility in the Context of Cell Therapy Biosafety.International journal of molecular sciences · 2023Review
- Generation of High-Yield, Functional Oligodendrocytes from a c-International journal of molecular sciences · 2021Article
- Genipin and EDC crosslinking of extracellular matrix hydrogel derived from human umbilical cord for neural tissue repair.Scientific reports · 2019Article
- Transplantation of neural precursors generated from spinal progenitor cells reduces inflammation in spinal cord injury via NF-κB pathway inhibition.Journal of neuroinflammation · 2019Article
- A Comparative Study of Three Different Types of Stem Cells for Treatment of Rat Spinal Cord Injury.Cell transplantation · 2017Article
- Human Umbilical Cord Blood-Derived Neural Stem Cell Line as a Screening Model for Toxicity.Neurotoxicity research · 2017Article
- Erasure and reestablishment of random allelic expression imbalance after epigenetic reprogramming.RNA (New York, N.Y.) · 2016Article
- Characterisation of neurons derived from a cortical human neural stem cell line CTX0E16.Stem cell research & therapy · 2015Article
- Single nucleotide polymorphism in the neuroplastin locus associates with cortical thickness and intellectual ability in adolescents.Molecular psychiatry · 2015Article
- Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS.Frontiers in cell and developmental biology · 2014Article
- The combination of mesenchymal stem cells and a bone scaffold in the treatment of vertebral body defects.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2013Article
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe use of immortalized neural stem cells either as models of neural development in vitro or as cellular therapies in central nervous system (CNS) disorders has been controversial. This controversy has centered on the capacity of immortalized cells to retain characteristic features of the progenitor cells resident in the tissue of origin from which they were derived, and the potential for tumorogenicity as a result of immortalization. Here, we report the generation of conditionally immortalized neural stem cell lines from human fetal spinal cord tissue, which addresses these issues.
methodsClonal neural stem cell lines were derived from 10-week-old human fetal spinal cord and conditionally immortalized with an inducible form of cMyc. The derived lines were karyotyped, transcriptionally profiled by microarray, and assessed against a panel of spinal cord progenitor markers with immunocytochemistry. In addition, the lines were differentiated and assessed for the presence of neuronal fate markers and functional calcium channels. Finally, a clonal line expressing eGFP was grafted into lesioned rat spinal cord and assessed for survival, differentiation characteristics, and tumorogenicity.
resultsWe demonstrate that these clonal lines (a) retain a clear transcriptional signature of ventral spinal cord progenitors and a normal karyotype after extensive propagation in vitro, (b) differentiate into relevant ventral neuronal subtypes with functional T-, L-, N-, and P/Q-type Ca(2+) channels and spontaneous calcium oscillations, and (c) stably engraft into lesioned rat spinal cord without tumorogenicity.
conclusionsWe propose that these cells represent a useful tool both for the in vitro study of differentiation into ventral spinal cord neuronal subtypes, and for examining the potential of conditionally immortalized neural stem cells to facilitate functional recovery after spinal cord injury or disease.
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