ArticleBMC genomics2013
Phenotypic, genomic, and transcriptional characterization of Streptococcus pneumoniae interacting with human pharyngeal cells.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- Article
- Streamlining marker-less allelic replacement inApplied and environmental microbiology · 2024Article
- Targeted control of pneumolysin production by a mobile genetic element inMicrobial genomics · 2022Article
- Secondary infection withFEMS microbes · 2022Article
- Time-resolved mRNA and miRNA expression profiling reveals crucial coregulation of molecular pathways involved in epithelial-pneumococcal interactions.Immunology and cell biology · 2020Article
- Rapamycin treatment correlates changes in primary cilia expression with cell cycle regulation in epithelial cells.Biochemical pharmacology · 2020Article
- Interacting networks of resistance, virulence and core machinery genes identified by genome-wide epistasis analysis.PLoS genetics · 2017Article
- Time-resolved dual RNA-seq reveals extensive rewiring of lung epithelial and pneumococcal transcriptomes during early infection.Genome biology · 2016Article
- Article
- Bright fluorescent Streptococcus pneumoniae for live-cell imaging of host-pathogen interactions.Journal of bacteriology · 2015Article
- The roles of transition metals in the physiology and pathogenesis of Streptococcus pneumoniae.Frontiers in cellular and infection microbiology · 2013Review
- The accessory Sec system (SecY2A2) in Streptococcus pneumoniae is involved in export of pneumolysin toxin, adhesion and biofilm formation.Microbes and infectionArticle
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
backgroundStreptococcus pneumoniae is a leading cause of childhood morbidity and mortality worldwide, despite the availability of effective pneumococcal vaccines. Understanding the molecular interactions between the bacterium and the host will contribute to the control and prevention of pneumococcal disease.
resultsWe used a combination of adherence assays, mutagenesis and functional genomics to identify novel factors involved in adherence. By contrasting these processes in two pneumococcal strains, TIGR4 and G54, we showed that adherence and invasion capacities vary markedly by strain. Electron microscopy showed more adherent bacteria in association with membranous pseudopodia in the TIGR4 strain. Operons for cell wall phosphorylcholine incorporation (lic), manganese transport (psa) and phosphate utilization (phn) were up-regulated in both strains on exposure to epithelial cells. Pneumolysin, pili, stress protection genes (adhC-czcD) and genes of the type II fatty acid synthesis pathway were highly expressed in the naturally more invasive strain, TIGR4. Deletion mutagenesis of five gene regions identified as regulated in this study revealed attenuation in adherence. Most strikingly, ∆SP_1922 which was predicted to contain a B-cell epitope and revealed significant attenuation in adherence, appeared to be expressed as a part of an operon that includes the gene encoding the cytoplasmic pore-forming toxin and vaccine candidate, pneumolysin.
conclusionThis work identifies a list of novel potential pneumococcal adherence determinants.
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