Evidence map›Paper›PMID 23758278›Full record

ArticleStem cells and development2013

The balance of positive and negative effects of TGF-β signaling regulates the development of hematopoietic and endothelial progenitors in human pluripotent stem cells.

Hao Bai, Yin-Liang Xie, Yong-Xing Gao, Tao Cheng, Zack Z Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. TGF-β signaling in health, disease, and therapeutics.Signal transduction and targeted therapy · 2024
    Review
  5. Article
  6. Review
  7. Regulation of Embryonic Stem Cell Self-Renewal.Life (Basel, Switzerland) · 2022
    Review
  8. Article
  9. hPSC-derived organoids: models of human development and disease.Journal of molecular medicine (Berlin, Germany) · 2021
    Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Vascular precursor cells in tissue injury repair.Translational research : the journal of laboratory and clinical medicine · 2017
    Review
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Hao Bai1 Division of Hematology, Johns Hopkins University School of Medicine , Baltimore, Maryland.
Yin-Liang Xie
Yong-Xing Gao
Tao Cheng
Zack Z Wang
Johns Hopkins University · USChinese Academy of Medical Sciences & Peking Union Medical College · CN

Funding

Functional Genomics of Platelet Aggregation Using iPS and Derived MegakaryocytesU01HL107446 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI BECKER, LEWIS C, CHENG, LINZHAO · 2011 to 2015
$8.8M
NHLBI NIH HHS U01 HL107446
6 · The paper itself

Abstract

Derived from mesoderm precursors, hemangioblasts are bipotential common progenitors of hematopoietic cells and endothelial cells. The regulatory events controlling hematopoietic and endothelial lineage specification are largely unknown, especially in humans. In this study, we establish a serum-free and feeder-free system with a high-efficient embryoid body (EB) generation to investigate the signals that direct differentiation of human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs). Consistent with previous studies, the CD34(+)CD31(+)VE-cadherin(+) (VEC(+)) cells derived from hPSCs contain hematopoietic and endothelial progenitors. In the presence of hematopoietic and endothelial growth factors, some of CD34(+)CD31(+)VEC(+) cells give rise to blast colony-forming cells (BL-CFCs), which have been used to characterize bipotential hemangioblasts. We found that the level of the transforming growth factor beta (TGF-β) 1 protein is increased during hPSC differentiation, and that TGF-β signaling has the double-edged effect on hematopoietic and endothelial lineage differentiation in hPSCs. An addition of TGF-β to hPSC differentiation before mesoderm induction promotes the development of mesoderm and the generation of CD34(+)CD31(+)VEC(+) cells. An addition of TGF-β inhibitor, SB431542, before mesoderm induction downregulates the expression of mesodermal markers and reduces the number of CD34(+)CD31(+)VEC(+) progenitor cells. However, inhibition of TGF-β signaling after mesoderm induction increases CD34(+)CD31(+)VEC(+) progenitors and BL-CFCs. These data provide evidence that a balance of positive and negative effects of TGF-β signaling at the appropriate timing is critical, and potential means to improve hematopoiesis and vasculogenesis from hPSCs.

Indexed as

BenzamidesCell DifferentiationCell LineageCell ProliferationCoculture TechniquesDioxolesEmbryoid BodiesEmbryonic DevelopmentEndothelial CellsGene Expression RegulationHematopoietic Stem CellsHumansInduced Pluripotent Stem CellsLymphocyte ActivationMesodermPrimary Cell Culture4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamideBenzamidesDioxolesTransforming Growth Factor beta1

Identifiers

PMID23758278
PMCPMC3787486
OpenAlexW1996572030

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.